Susceptibility of a field-derived, Bacillus thuringiensis-resistant strain of diamondback moth to in vitro-activated Cry1Ac toxin
Susceptibility of a field-derived, Bacillus thuringiensis-resistant strain of diamondback moth to in vitro-activated Cry1Ac toxin
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DOI：
10.1128/aem.67.9.4372-4373.2001
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发表时间：
2001-09-01
影响因子：
4.4
通讯作者：
Crickmore, N
中科院分区：
文献类型：
--
作者：
Sayyed, AH;Gatsi, R;Crickmore, N
用结晶、溶解、部分和完全活化形式的苏云金芽孢杆菌Cry 1Ac δ-内毒素测试小菜蛾（Plutella xylostella）的抗性和敏感种群。完全活化的毒素大大降低了抗性种群的抗性比（抗性种群的50%致死浓度与敏感种群的50%致死浓度之比），表明毒素活化缺陷是主要的抗性机制。
Resistant and susceptible populations of the diamondback moth (Plutella xylostella) were tested with crystalline, solubilized, and partially and fully activated forms of the Bacillus thuringiensis Cry1Ac delta -endotoxin. Fully activated toxin greatly reduced the resistance ratio (ratio of the 50% lethal concentration for the resistant population to that for the susceptible population) of the resistant population, suggesting that a defect in toxin activation is a major resistance mechanism.