PREPARATION OF HIGH-DENSITY LIPOPROTEINS LABELED EXCLUSIVELY AT THE CHOLESTERYL ESTER MOIETY
PREPARATION OF HIGH-DENSITY LIPOPROTEINS LABELED EXCLUSIVELY AT THE CHOLESTERYL ESTER MOIETY
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DOI：
10.1016/0009-8981(82)90122-x
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发表时间：
1982-01-01
影响因子：
5
通讯作者：
ASSMANN, G
中科院分区：
文献类型：
--
作者：
SCHRIEWER, H;JABS, HU;ASSMANN, G
胆固醇酯部分放射性标记的[人] HDL [高密度脂蛋白]的体外制备通过将不同血清组分与溶于二甲基亚砜（DMSO）的[3 H]胆固醇亚油酸酯或胆固醇[14 C]油酸酯孵育，随后通过超离心从孵育混合物中重新分离HDL来测定。使用通过用磷钨酸盐/MgCl 2沉淀含载脂蛋白B的脂蛋白获得的上清液或使用> 1.075 kg/l的血清部分，可以在再分离的HDL部分中回收大部分添加的放射性，如密度梯度超离心或凝胶色谱法所示。使用全血清或> 1.063 kg/l血清部分的孵育实验导致标记的胆固醇亚油酸酯与含载脂蛋白B的脂蛋白优先缔合。
The in vitro preparation of [human] HDL [high density lipoprotein] radiolabeled at the cholesteryl ester moiety was determined by incubating different serum fractions with [3H]cholesteryl linoleate or cholesteryl [14C]oleate dissolved in dimethylsulfoxide (DMSO) and subsequent reisolation of HDL from the incubation mixture by ultracentrifugation. Using the supernatant obtained by precipitation of apolipoprotein B-containing lipoproteins with phosphotungstate/MgCl2 or using the > 1.075 kg/l fraction of sera, the bulk of the added radioactivity could be recovered in the reisolated HDL fraction, as demonstrated by density gradient ultracentrifugation or by gel chromatography. Incubation experiments using whole sera or the > 1.063 kg/l fraction of sera resulted in the preferential association of the labeled cholesteryl linoleate with apolipoprotein B-containing lipoproteins.