Determination of Polyhydroxy Antibiotics by Capillary Electrophoresis with Amperometric Detection at a Copper Electrode
Determination of Polyhydroxy Antibiotics by Capillary Electrophoresis with Amperometric Detection at a Copper Electrode
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发表时间：
1995
影响因子：
3.1
通讯作者：
Fang Yu
中科院分区：
文献类型：
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作者：
Fang Yu
采用毛细管电泳（CE）在碱性介质中分离多羟基抗生素，并在铜电极上进行电化学检测。所用电极为直径350 μm的Cu盘，采用壁喷射结构。在30 min内分离了7种多羟基抗生素，线性范围为2-3个数量级，检出限为0.5 μmol/L，每小时进样50 μ mol/L双霉素，在8 h内峰电流和迁移时间的重现性分别为7.6%和2.2%。对真实的样品测定的回收率为99.6%。
Capillary electrophoresis(CE) was employed to separate polyhydroxy antibiotics in alkaline solution, combined with electrochemical detection at copper electrode. The electrode used was a 350 μm diameter Cu disk operated in a wall-jet configuration. A sample mixture containing 7 different polyhydroxy antibibeics was separated within 30 min. The linearity varied over a 2-3 order of magnitude and the detection limits were 0.5 μmol/L.The reproducibility for the peak current and the migration time of 50 μ mol/L doublemycin injected every hour in a period of 8h, was 7.6%and 2.2%RSD, respectively, and the recovery of the determination of the real sample was 99.6%.