REGULATION OF ALTERNATIVE PRE-MESSENGER-RNA SPLICING BY A NOVEL REPEATED HEXANUCLEOTIDE ELEMENT
REGULATION OF ALTERNATIVE PRE-MESSENGER-RNA SPLICING BY A NOVEL REPEATED HEXANUCLEOTIDE ELEMENT
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DOI：
10.1101/gad.8.13.1561
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发表时间：
1994-07-01
影响因子：
10.5
通讯作者：
HYNES, RO
中科院分区：
文献类型：
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作者：
HUH, GS;HYNES, RO
选择性剪接外显子EIIIB在大鼠纤连蛋白基因中以细胞类型特异性方式调节。EIIIB剪接成纤连蛋白mRNA依赖于紧邻EIIIB下游的内含子中的序列。我们发现，一个短的，高度重复的TGCATG基序在这个内含子是重要的细胞类型特异性识别EIIIB作为一个外显子。该基序增强了EIIIB 5'剪接位点的使用;此外，该重复的TGCATG序列可以激活不相关的大鼠前速激肽原前体mRNA中的可变剪接外显子。有趣的是，这个序列也可以在其他选择性剪接基因中发现的顺式作用元件中找到。因此，这种短重复TGCATG基序是细胞类型特异性元件，除了控制纤连蛋白选择性剪接外，还可能参与其他选择性RNA加工事件的调节。
The alternatively spliced exon EIIIB is regulated in a cell type-specific manner in the rat fibronectin gene. Splicing of EIIIB into fibronectin mRNA is dependent on sequences in the intron immediately downstream of EIIIB. We show that a short, highly repeated TGCATG motif in this intron is important for cell type-specific recognition of EIIIB as an exon. This motif enhances usage of the EIIIB 5' splice site; furthermore, this repeated TGCATG sequence can activate an alternatively spliced exon in the unrelated rat preprotachykinin pre-mRNA. Interestingly, this sequence can also be found within cis-acting elements identified previously in other alternatively spliced genes. This short repeated TGCATG motif is therefore a cell type-specific element that, in addition to controlling fibronectin alternative splicing, may participate in the regulation of other alternative RNA processing events.