Uptake of l‐Glutamate and Taurine in Neuroblastoma Cells with Altered Fatty Acid Composition of Membrane Phospholipids
Uptake of l‐Glutamate and Taurine in Neuroblastoma Cells with Altered Fatty Acid Composition of Membrane Phospholipids
复制标题
膜磷脂脂肪酸组成改变的神经母细胞瘤细胞对 L-谷氨酸和牛磺酸的摄取
DOI：
10.1111/j.1471-4159.1980.tb11260.x
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发表时间：
1980
影响因子：
4.7
通讯作者：
P. Mandel
中科院分区：
文献类型：
--
作者：
V. Balcar;J. Borg;J. Robert;P. Mandel
摘要：在已建立的神经母细胞瘤细胞系M1中，研究了膜脂组成改变对高亲和力摄取L-谷氨酸和牛磺酸的影响。作为膜脂组分的某些脂肪酸(20：5ω3和22：5ω3)的参与程度增加，导致L谷氨酸摄取的最大初始速率(Vmax)增加4倍，而牛磺酸摄取的Vmax的增加幅度要小得多。膜对L-谷氨酸摄取的结构要求和对L-谷氨酸或牛磺酸的被动通透性似乎不受脂质组成变化的影响。由于膜磷脂中20：5ω3和22：5ω3的比例增加并未引起牛磺酸摄取动力学参数的显著变化，并且亚油酸或亚麻酸单独掺入磷脂中对某些测量参数的影响较小，因此讨论了22：5ω3和/或20：5ω3与L谷氨酸摄取之间存在特定关系的可能性。与其他制剂中的L-谷氨酸摄取系统不同，神经母细胞瘤细胞中L-谷氨酸的高亲和力摄取系统不容易接受L-天冬氨酸作为底物。
Abstract: Effects of altered composition of membrane lipids on high‐affinity uptake of l‐glutamate and taurine were studied in an established neuroblastoma cell line M1. Increase in participation of certain fatty acids (20: 5ω3 and 22: 5ω3) as constituents of membrane lipids resulted in a fourfold increase in the maximum initial rate of l‐glutamate uptake (Vmax) while increase in Vmax of taurine uptake was much smaller. Neither structural requirements of l‐glutamate uptake nor passive permeability of the membrane to l‐glutamate or taurine seemed to be influenced by the alterations in the lipid composition. Since increased proportions of 20: 5ω3 and 22: 5ω3 in the membrane phosphatides caused no dramatic changes in kinetic parameters of taurine uptake and incorporation of either linoleic or linolenic acid alone into the phosphatides had only a relatively small effect on some of the measured parameters, the possibility of a specific relationship between 22: 5ω3 and/or 20: 5ω3 and l‐glutamate uptake is discussed. Unlike l‐glutamate uptake systems in other preparations, the high‐affinity uptake system of l‐glutamate in neuroblastoma cells did not readily accept l‐aspartate as a substrate.