Differential Impact of IL-10 Expression on Survival and Relapse between HPV16-Positive and -Negative Oral Squamous Cell Carcinomas
Differential Impact of IL-10 Expression on Survival and Relapse between HPV16-Positive and -Negative Oral Squamous Cell Carcinomas
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DOI：
10.1371/journal.pone.0047541
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发表时间：
2012-10-31
期刊：
影响因子：
3.7
通讯作者：
Lee, Huei
中科院分区：
文献类型：
--
作者：
Chuang, Chun-Yi;Sung, Wen-Wei;Lee, Huei
人乳头瘤病毒（HPV）是一个危险因素，在一个子集的口咽癌;然而，HPV的贡献，在恶性口腔鳞状细胞癌（OSCC）尚未完全了解在台湾。在此，61例无风险因素的患者和117例有一个或多个风险因素的患者入组本研究。HPV 16/18感染率在不吸烟、不饮酒、不嚼槟榔人群中较高。HPV感染的癌症的发展已被证明与白细胞介素-10（IL-10）表达相关。为此，IL-10 mRNA在口腔鳞癌肿瘤中的表达通过实时RT-PCR进行评估。数据显示，HPV阳性患者的IL-10 mRNA水平高于HPV阴性患者。Kaplan-Meier和Cox回归分析显示，IL-10 mRNA仅在HPV阳性的口腔鳞癌中对总生存期和无复发生存期有预测意义，而在HPV阴性的口腔鳞癌中无意义。从机制上讲，E6升高IL-10是导致OSCC细胞集落形成和迁移能力增加的原因。因此，我们认为E6诱导的IL-10促进细胞生长和迁移能力，从而降低HPV阳性口腔鳞癌的生存率和复发率。
Human papillomavirus (HPV) is a risk factor in a subset of oropharyngeal cancer; however, the contribution of HPV in the malignancy of oral squamous cell carcinomas (OSCC) is not fully understood in Taiwanese. Herein, 61 patients with no risk factors and 117 patients with one or more risk factors were enrolled in this study. HPV16/18 infection rate in non-smokers, non-drinkers and non-betel quid chewers was higher than their counterparts. The development of HPV-infected cancer has been shown to be associated with interleukin-10 (IL-10) expression. To this end, IL-10 mRNA expression in OSCC tumors was evaluated by real-time RT-PCR. Data showed that HPV-positive patients had higher IL-10 mRNA levels than in HPV-negative patients. Kaplan-Meier and Cox-regression analysis indicated that the prognostic significance of IL-10 mRNA on overall survival and relapse free survival was only observed in HPV-positive OSCC, but not in HPV-negative OSCC. Mechanistically, the elevation of IL-10 by E6 was responsible for increased colony formation and migration capability in OSCC cells. Therefore, we suggest that IL-10 induced by E6 promotes cell growth and migration capability and consequent poor survival and relapse in HPV-positive OSCC.