An extracellular aminopeptidase encoded by the ywaD gene plays an important role in supplying nitrogen nutrition for the growth of Bacillus subtilis 168
An extracellular aminopeptidase encoded by the ywaD gene plays an important role in supplying nitrogen nutrition for the growth of Bacillus subtilis 168
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ywaD基因编码的胞外氨肽酶在为枯草芽孢杆菌生长提供氮营养方面发挥着重要作用168
DOI：
10.1139/cjm-2016-0602
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发表时间：
2017
影响因子：
2.8
通讯作者：
Zhou Zhemin
中科院分区：
文献类型：
--
作者：
Liu Zhongmei;Gao Xinxing;Zhou Li;Cui Wenjing;Tian Yaping;Zhou Zhemin
为了研究枯草芽孢杆菌（Bacillus subtilis 168）theywaD基因编码的胞外氨基肽酶（BSAP 168）的生理功能，我们构建了枯草芽孢杆菌（Bacillus subtilis 168）theywaD基因缺失突变株（BS-AP-K）。与野生型菌株相比，BS-AP-K在37 °C大豆蛋白培养基中的最大生长速率降低了28%，而在Luria-Bertani培养基中没有降低。在较高的温度下，生长速率受损更明显，并且可以通过向培养基中补充氨基酸来补偿。进一步的研究表明，野生型菌株和BS-AP-K菌株培养上清中的氨基酸组成和肽分布存在明显差异。此外，通过对ΔPA进行双酶切，构建了BSAP 168突变株（BS-AP-R），以研究BSAP 168蛋白酶相关结构域对生长的影响。这些结果表明BSAP 168在提供生长所需的氨基酸方面起着重要作用。
To investigate the physiological role of an extracellular aminopeptidase (BSAP168) encoded by theywaDgene inBacillus subtilis168, we constructed theywaD-deletion mutant (BS-AP-K). Compared with that of the wild-type strain, the maximum growth rate of BS-AP-K was reduced by 28% when grown in soybean protein medium at 37 °C, but not in Luria–Bertani medium. The impaired growth rate was more marked at higher temperature and could be compensated by supplementation of amino acid to the culture media. Further studies showed that in regards to the amino acid compositions and peptide distribution in the culture supernatants, there was an obvious difference between the culture supernatants of wild-type and BS-AP-K strains. In addition, another mutant strain (BS-AP-R) was constructed by replacingywaDwithywaD-ΔPA to evaluate the effect of a protease-associated domain in BSAP168 on growth. All these findings indicated that BSAP168 played an important role in supplying the amino acids required for growth.