Advances in on-chip photodetection for applications in miniaturized genetic analysis systems
Advances in on-chip photodetection for applications in miniaturized genetic analysis systems
复制标题
DOI：
10.1088/0960-1317/14/1/311
复制
发表时间：
2004-01-01
影响因子：
2.3
通讯作者：
Burns, MA
中科院分区：
文献类型：
--
作者：
Namasivayam, V;Lin, RS;Burns, MA
微加工技术在下一代DNA分析设备的开发中变得越来越流行。改进的芯片上荧光检测系统可以应用于开发用于护理点诊断的便携式手持仪器。荧光检测的小型化涉及超灵敏光电检测器的构建，其可以集成到与适当的光学发射滤波器组合的流体平台上。我们先前已经展示了集成PIN光电二极管到微加工电泳通道的DNA片段的分离和检测。在这项工作中，我们提出了一种改进的检测器结构，使用PINN+光电二极管与片上干涉滤光片和一个强大的液体阻挡层。这种新的设计在整个可见光谱范围内产生高灵敏度（检测限为0.9 ng穆尔（-1）DNA）、低噪声（S/N类似于100/1）和增强的量子效率（> 80%）。这些光电二极管在DNA分析的各个领域，如微反应（PCR），分离（电泳）和微流体（液滴传感）的应用。
Microfabrication techniques have become increasingly popular in the development of next generation DNA analysis devices. Improved on-chip fluorescence detection systems may have applications in developing portable hand-held instruments for point-of-care diagnostics. Miniaturization of fluorescence detection involves construction of ultra-sensitive photodetectors that can be integrated onto a fluidic platform combined with the appropriate optical emission filters. We have previously demonstrated integration PIN photodiodes onto a microfabricated electrophoresis channel for separation and detection of DNA fragments. In this work, we present an improved detector structure that uses a PINN+ photodiode with an on-chip interference filter and a robust liquid barrier layer. This new design yields high sensitivity (detection limit of 0.9 ng mul(-1) of DNA), low-noise (S/N similar to 100/1) and enhanced quantum efficiencies (> 80%) over the entire visible spectrum. Applications of these photodiodes in various areas of DNA analysis such as microreactions (PCR), separations (electrophoresis) and microfluidics (drop sensing) are presented.