Separation and mapping of chromosomes of parasitic protozoa
Separation and mapping of chromosomes of parasitic protozoa
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DOI：
10.1590/s0074-02761997000600017
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发表时间：
1997-11-01
影响因子：
2.8
通讯作者：
Scherf, A
中科院分区：
文献类型：
--
作者：
HernandezRivas, R;Scherf, A
Many protozoan parasites represent an important group of human pathogens. Pulsed Field Gradient Gel Electrophoresis (PFGE) analysis has been an important tool for fundamental genetic studies of parasites like Trypanosoma, Leishmania, Giardia or the human malaria parasite Plasmodium falciparum. We present PFGE conditions allowing a high resolution separation of chromosomes ranging from 500 to 4000 kb within a two day electrophoresis run. In addition, we present conditions for separating large chromosomes (2000-6000 kb) within 36 hi: We demontrate that the application of two dimentional PFGE (2D-PFGE) technique to parasite karyotypes is a very useful method for the analysis of dispersed gene families and comparative studies of the intrachomosomal genome organization.
