Iodination of biological samples without loss of functional activity.
Iodination of biological samples without loss of functional activity.
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生物样品的碘化而不损失功能活性。
DOI：
10.1016/0003-2697(82)90156-7
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发表时间：
1982
影响因子：
2.9
通讯作者：
J. Ballesta
中科院分区：
文献类型：
--
作者：
F. Tejedor;J. Ballesta
提出了一种通过对敏感生物材料进行碘化处理的放射性标记技术，该技术比标准的碘化方法在更大程度上保留了样品的功能活性。反应是保持底物和产碘系统在不同的相中进行的。氯胺- t在水和Cl -离子存在下，在靠近含有底物和125i -的溶液表面的一张滤纸上产生cl2。cl2分子从纸上扩散，进入溶液，并与碘离子反应，生成改变样品芳香环的碘。在这些碘化条件下处理的蛋白A、溶菌酶和核糖体的活性受到的影响比用标准氯胺- t方法或碘化系统碘化时要小得多。此外，这种技术，我们称之为“两相”系统，似乎优先作用于结构的表面，研究核糖体蛋白的碘化模式表明。
A technique for radioactive labeling by iodination of sensitive biological material that preserves the functional activity of the samples to a greater extent than the standard iodination methods is presented. The reaction is carried out keeping in separate phases the substrate and the iodine-generating system. Chloramine-T in the presence of water and Cl−ions generates Cl2on a piece of filter paper kept close to the surface of the solution containing the substrate and the125I−. The Cl2molecules diffuse from the paper, enter the solution, and, reacting with the iodide ions, generate iodine that modifies the aromatic rings of the sample. Protein A, lysozyme, and ribosomes treated under these iodination conditions are much less affected in their activity than when iodinated by the standard chloramine-T method or the iodogen system. In addition, this technique, which we have called “two phases” system, seems to act preferentially on the surface of the structures as shown by studying the iodination pattern of the ribosomal proteins.
放射性标记的高纯度牛促甲状腺素的生物活性研究。
DOI：
10.1016/0006-291x(80)90301-0
发表时间：
1980
影响因子：
3.1
作者：
Takai,NA;Filetti,S;Rapoport,B
通讯作者：
Rapoport,B