Genes determining pathogenicity to pea are clustered on a supernumerary chromosome in the fungal plant pathogen Nectria haematococca
Genes determining pathogenicity to pea are clustered on a supernumerary chromosome in the fungal plant pathogen Nectria haematococca
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DOI：
10.1046/j.1365-313x.2001.00969.x
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发表时间：
2001-02-01
期刊：
影响因子：
7.2
通讯作者：
VanEtten, HD
中科院分区：
文献类型：
--
作者：
Han, YN;Liu, XG;VanEtten, HD
三个基因，有助于真菌赤壳菌的能力，导致疾病的豌豆植物已被确定。这些豌豆致病性（PEP）基因是在25 kb内的彼此和位于一个额外的染色体。总之，PEP基因簇包含六个转录单位，在豌豆组织感染过程中表达。只有一个基因的生化功能是确定的。这个基因，PDA 1，编码一种特殊的细胞色素P450，赋予抗pisatin，豌豆植物产生的抗生素。三个新的PEP基因，除了PDA 1，可以独立地增加真菌的能力，造成豌豆病变时，加入到一个分离缺乏额外染色体。基于预测的氨基酸序列，假设这三个基因中的两个的功能。PEP簇的另一个转录部分的推导的氨基酸序列，以及簇中的其他四个开放阅读框，与已知的真菌转座酶具有高度的相似性。PEP簇的几个特征-致病性基因簇、转座因子的存在以及密码子使用和GC含量与基因组其他部分的差异-被植物和动物的致病性细菌中的致病性岛共享。
Three genes that contribute to the ability of the fungus Nectria haematococca to cause disease on pea plants have been identified. These pea pathogenicity (PEP) genes are within 25 kb of each other and are located on a supernumerary chromosome. Altogether, the PEP gene cluster contains six transcriptional units that are expressed during infection of pea tissue. The biochemical function of only one of the genes is known with certainty. This gene, PDA1, encodes a specific cytochrome P450 that confers resistance to pisatin, an antibiotic produced by pea plants. The three new PEP genes, in addition to PDA1, can independently increase the ability of the fungus to cause lesions on pea when added to an isolate lacking the supernumerary chromosome. Based on predicted amino acid sequences, functions for two of these three genes are hypothesized. The deduced amino acid sequence of another transcribed portion of the PEP cluster, as well as four other open reading frames in the cluster, have a high degree of similarity to known fungal transposases. Several of the features of the PEP cluster - a cluster of pathogenicity genes, the presence of transposable elements, and differences in codon usage and GC content from other portions of the genome - are shared by pathogenicity islands in pathogenic bacteria of plants and animals.