Are nitric oxide donors a valuable tool to study the functional role of nitric oxide in plant metabolism?
Are nitric oxide donors a valuable tool to study the functional role of nitric oxide in plant metabolism?
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DOI：
10.1111/j.1438-8677.2010.00430.x
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发表时间：
2011-09-01
期刊：
影响因子：
3.9
通讯作者：
Kosmala, A.
中科院分区：
文献类型：
--
作者：
Arasimowicz-Jelonek, M.;Floryszak-Wieczorek, J.;Kosmala, A.
在目前的工作中，我们测试了已知的一氧化氮（NO）调节剂产生NO+（硝普钠，SNP）和NO中心点形式（S-亚硝基-N-乙酰基-D-青霉胺，SNAP和亚硝基谷胱甘肽，GSNO）。这使我们能够比较下游NO相关的生理效应的蛋白质中发现的天竺葵（天竺葵peltatum L.）。通过NO供体的蛋白质修饰通常以不同的方式影响植物代谢，表现为响应于SNAP和GSNO的较低的硫代巴比妥酸反应物质（TBARS）含量和脂氧合酶（LOX）活性。这与SNP治疗观察到的反应相反。大多数酶活性（GR，谷胱甘肽还原酶; GST，谷胱甘肽-S-转移酶; GPX，谷胱甘肽过氧化物酶）的变化在孵育的前8小时内最为显著和可重复，这可以通过所用供体的半衰期来解释。特别是，一个密切的依赖关系被发现之间的时间过程中的NO排放从应用的捐助者和暂时抑制的抗氧化酶，如过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）。所观察到的变化伴随着蛋白质积累的时间依赖性的改变，通过二维凝胶电泳（2-DE）分析，在天竺葵叶片处理NO供体（SNP，SNAP和GSNO）。使用蛋白质组学，发现不同的蛋白质被下调和上调。然而，没有新的蛋白质斑点特征的所有三个donors. These结果表明，NO的形式发射的供体结构中起着关键作用，在适当的代谢修饰开关。已经注意到，由所用供体诱导的几种NO影响的代谢组学变化不具有可比性，这证实了在解释使用不同NO调节剂化合物的药理学方法获得的结果时需要保持谨慎。
In the present work, we tested known nitric oxide (NO) modulators generating the NO+ (sodium nitroprusside, SNP) and NO center dot forms (S-nitroso-N-acetyl-D-penicillamine, SNAP and nitrosoglutathione, GSNO). This allowed us to compare downstream NO-related physiological effects on proteins found in leaves of pelargonium (Pelargonium peltatum L.). Protein modification via NO donors generally affects plant metabolism in a distinct manner, manifested by a lower thiobarbituric acid reactive substance (TBARS) content and lipoxygenase (LOX) activity in response to SNAP and GSNO. This is in contrast to the response observed for SNP treatment. Most changes in enzyme activity (GR, glutathione reductase; GST, glutathione-S-transferase; GPX, glutathione peroxidase) are most spectacular and repeatable during the first 8 h of incubation, which is explained by the half-life of the applied donors. In particular, a close dependence was found between the time-course of NO emission from the applied donors and the temporary inhibition of antioxidant enzymes, such as catalase (CAT) and ascorbate peroxidase (APX). The observed changes were accompanied by time-dependent alterations in protein accumulation as analysed by two-dimensional gel electrophoresis (2-DE) in pelargonium leaves treated with NO donors (SNP, SNAP and GSNO). Using proteomics, different proteins were found to be down-and up-regulated. However, no new protein spots characteristic of all three donors were found. These results indicate that the form of NO emitted from the donor structure plays a key role in switching on appropriate metabolic modifications. It has been noted that several NO-affected metabolomic changes induced by the used donors were not comparable, which confirms the need to maintain caution when interpreting results obtained using the pharmacological approach with different NO modulator compounds.