PrPSc accumulation in myocytes from sheep incubating natural scrapie
PrPSc accumulation in myocytes from sheep incubating natural scrapie
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DOI：
10.1038/nm1055
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发表时间：
2004-06-01
期刊：
影响因子：
82.9
通讯作者：
Schelcher, F
中科院分区：
文献类型：
--
作者：
Andréoletti, O;Simon, S;Schelcher, F
由于人类的变异型克雅氏病(VCJD)可能是由于食用了受牛海绵状脑病动物组织污染的产品造成的，因此反刍动物肌肉中是否存在传染性普恩蛋白是一个关键问题。在这里，我们展示了实验上和自然感染瘙痒病的绵羊在一个肌细胞亚群中积累了PrPSc蛋白。在自然感染的绵羊中，在临床疾病发作前几个月就可以在肌肉中检测到PrPSc。PrPSc的相对含量表明，与大脑相比，肌肉的传染性低5000倍。
Because variant Creutzfeldt-Jakob disease (vCJD) in humans probably results from consumption of products contaminated with tissue from animals with bovine spongiform encephalopathy, whether infectious prion protein is present in ruminant muscles is a crucial question. Here we show that experimentally and naturally scrapie-affected sheep accumulate the prion protein PrPSc in a myocyte subset. In naturally infected sheep, PrPSc is detectable in muscle several months before clinical disease onset. The relative amounts of PrPSc suggest a 5,000-fold lower infectivity for muscle as compared to brain.