A Novel Label-Free Immunosensor Based on Activated Graphene Oxide for Acetaminophen Detection
A Novel Label-Free Immunosensor Based on Activated Graphene Oxide for Acetaminophen Detection
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DOI：
10.1002/elan.201400583
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发表时间：
2015-03
期刊：
影响因子：
3
通讯作者：
M. Tertiș;O. Hosu;L. Fritea;C. Farcǎu;Andreea Cernat;R. Sandulescu;C. Cristea
中科院分区：
文献类型：
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作者：
M. Tertiș;O. Hosu;L. Fritea;C. Farcǎu;Andreea Cernat;R. Sandulescu;C. Cristea
在1-乙基-3-（3-二甲基氨基丙基）碳二亚胺盐酸盐存在下，通过N-羟基琥珀酰亚胺修饰石墨基丝网印刷电极，制备了一种用于选择性检测对乙酰氨基酚的简单无标记免疫传感器。通过逐层技术制备的模板提供了活化的羧基，其与来自抗对乙酰氨基酚抗体的末端胺基形成ammonium共价键。采用电化学阻抗谱、循环伏安法和方波伏安法对抗体固定化和牛血清白蛋白对石墨烯模板上自由活性基团的封闭进行了优化。电化学石英晶体微天平技术用于确定在优化步骤期间沉积到电极表面上的不同组分的量。将该免疫传感器应用于合成样品和真实的样品中对乙酰氨基酚的测定，结果良好。获得的检测限为0.17 µM（S/N=3），剂型辅料和血清组分无干扰或干扰极小。
A simple label-free immunosensor for the selective detection of acetaminophen was developed by modifying a graphite based screen-printed electrode with graphene oxide after the functionalization with N-hydroxysuccinimide in the presence of 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride. The template made by layer-by-layer technique provides activated carboxylic groups, which form amidic covalent bonds with the terminal amine groups from the antiacetaminophen antibody. The optimization of antibody immobilization and the blocking of the free active groups from graphene template with bovine serum albumin was performed by using electrochemical impedance spectroscopy, cyclic voltammetry and square wave voltammetry. The electrochemical quartz crystal microbalance technique was used to determine the quantity of different components deposited onto the electrode surface during the optimization steps. The above described immunosensor was applied with good results for the determination of acetaminophen in synthetic and real samples by using square wave voltammetry. A limit of detection of 0.17 µM (S/N=3) was obtained with no or minimal interference of dosage forms excipients and serum components.