THE AUTOGRAPHA-CALIFORNICA BACULOVIRUS GENOME - EVIDENCE FOR MULTIPLE REPLICATION ORIGINS
THE AUTOGRAPHA-CALIFORNICA BACULOVIRUS GENOME - EVIDENCE FOR MULTIPLE REPLICATION ORIGINS
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DOI：
10.1126/science.1529337
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发表时间：
1992-09-04
期刊：
影响因子：
56.9
通讯作者：
ROHRMANN, G
中科院分区：
文献类型：
--
作者：
PEARSON, M;BJORNSON, R;ROHRMANN, G
加州签名虫多核衣壳核多角体病毒（AcMNPV）被用于真核生物基因的过表达，并被设计为可能用作病毒杀虫剂，它具有一个圆形的、超卷曲的基因组，大约有128千碱基。尽管它被广泛使用，但人们对AcMNPV的复制机制知之甚少。本报告提供的证据表明，DNA复制的AcMNPV起源是重复序列，每个序列包含几个密切相关的不完美回文，这些回文存在于分布在基因组周围的六个区域。虽然AcMNPV感染依赖的质粒复制是由一个完整的回文启动的，但在含有6个或8个回文的质粒中，复制的数量大大增加。
The Autographa californica multinucleocapsid nuclear polyhedrosis virus (AcMNPV), which is used for the overexpression of eukaryotic genes and is being engineered for possible use as a viral insecticide, has a circular, supercoiled genome of approximately 128 kilobases. Despite its widespread use, little is known about the mechanism by which AcMNPV replicates. Evidence is presented in this report that AcMNPV origins of DNA replication are repeated sequences each containing several closely related imperfect palindromes that are present in six regions distributed around the genome. Although AcMNPV infection-dependent plasmid replication was initiated by a single complete palindrome, the amount of replication was substantially increased in plasmids containing six or eight palindromes.