Localization of aquaporins 1, 2, and 3 and vasopressin type 2 receptor in the mouse inner ear
Localization of aquaporins 1, 2, and 3 and vasopressin type 2 receptor in the mouse inner ear
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DOI：
10.3109/00016489.2012.662718
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发表时间：
2012-07
影响因子：
1.4
通讯作者：
M. Takumida;A. Kakigi;N. Egami;R. Nishioka;M. Anniko
中科院分区：
文献类型：
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作者：
M. Takumida;A. Kakigi;N. Egami;R. Nishioka;M. Anniko
摘要结论：研究表明，血管纹、前庭暗细胞和移行细胞、内淋巴囊上皮细胞等液体运输细胞中的水通道蛋白（AQP）1、2和3以及加压素2型受体（V2 Rs）在内耳液体运输中发挥重要作用，而感觉细胞和神经节细胞中的AQP 1、2和3以及加压素2型受体（V2 Rs）可能在感觉细胞转导系统中发挥功能作用。目的：分析AQP 1、AQP 2、AQP 3以及V2 Rs在正常小鼠内耳中的表达。方法：CBA/J小鼠。AQP 1，AQP 2，AQP 3和V2 Rs在内耳，即耳蜗，前庭终器和内淋巴囊的定位通过免疫组织化学进行了研究。结果如下：结果表明，AQP 1、AQP 2、AQP 3和V2 Rs在内耳血管纹、内毛细胞、外毛细胞、螺旋神经节细胞、前庭感觉细胞、前庭神经节细胞、前庭暗细胞、前庭移行细胞和内淋巴囊等结构中均有丰富的分布。
Abstract Conclusion: It is suggested that aquaporins (AQPs) 1, 2, and 3, and vasopressin type 2 receptors (V2Rs) in the fluid transporting cells, such as stria vascularis, vestibular dark and transitional cells, and endolymphatic sac epithelial cells, have an important role in fluid transport in the inner ear, while those in the sensory and ganglion cells may play a functional role in the sensory cell transduction system. Objective: To analyze expression of AQP1, AQP2, and AQP3 as well as V2Rs in the normal mouse inner ear. Methods: CBA/J mice were used in this study. Localization of AQP1, AQP2, AQP3, and V2Rs in the inner ear, i.e. cochlea, vestibular end organs, and endolymphatic sac, was investigated by immunohistochemistry. Results: The results show that AQP1, AQP2, AQP3, and V2Rs are abundantly distributed in many inner ear structures, i.e. stria vascularis, inner and outer hair cells, spiral ganglion cells, vestibular sensory and ganglion cells, vestibular dark and transitional cells, and the endolymphatic sac.