The amphotropic murine leukemia virus receptor gene encodes a 71-kilodalton protein that is induced by phosphate depletion
The amphotropic murine leukemia virus receptor gene encodes a 71-kilodalton protein that is induced by phosphate depletion
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DOI：
10.1128/jvi.71.6.4564-4570.1997
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发表时间：
1997-06-01
影响因子：
5.4
通讯作者：
Garcia, JV
中科院分区：
文献类型：
--
作者：
Chien, ML;Foster, JL;Garcia, JV
嗜异性鼠白血病病毒（MuLV）可通过其特异性受体感染包括人在内的多种哺乳动物细胞，了解MuLV受体表达的基础知识有助于开发和改进基于嗜异性假型载体的基因治疗方案。（GLVR-2，新命名为Pit 2），我们在几个细胞系中测定了其mRNA水平，发现它们差异显着。在两种骨肉瘤细胞系中观察到从培养基中去除磷酸盐后mRNA水平增加的诱导。GLVR-2 mRNA的增加导致由针对GLVR-2的亲和纯化抗体特异性识别的71-kDa蛋白质水平的伴随升高。我们能够证实GLVR-2蛋白的第六和第七跨膜结构域之间的大亲水结构域的细胞内拓扑结构，该分配与第四胞外环位于细胞外一致，与GLVR-2的第四胞外环含有包膜结合位点的提议一致。
The amphotropic murine leukemia virus (MuLV) can infect cells from a number of mammals, including humans, via its specific receptor, Basic knowledge of amphotropic MuLV receptor expression is likely to be useful in the development and improvement of gene therapy protocols based on amphotropic-pseudotyped vectors, To investigate the expression of the human receptor for the amphotropic MuLV (GLVR-2, newly termed Pit2), we determined its mRNA levels in several cell lines and found them to vary significantly. Induction of increased levels of mRNA after removal of phosphate from the media was observed in two osteosarcoma cell lines, The increase in GLVR-2 mRNA resulted in a concomitant rise in the levels of a 71-kDa protein specifically recognized by affinity-purified antibodies against GLVR-2, Using these antibodies, we were able to confirm the intracellular topology of the large hydrophilic domain between the proposed sixth and seventh transmembrane domains of the GLVR-2 protein, This assignment is in agreement with the fourth extracellular loop being outside the cell, consistent with the proposal that the fourth extracellular loop of GLVR-2 contains the envelope binding site.