Biochemical changes during vitellogenesis in the red claw crayfish, Cherax quadricarinatus (von Martens)
Biochemical changes during vitellogenesis in the red claw crayfish, Cherax quadricarinatus (von Martens)
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DOI：
10.1111/j.1365-2109.2010.02493.x
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发表时间：
2010-09-16
影响因子：
2
通讯作者：
Zhao, Yunlong
中科院分区：
文献类型：
--
作者：
Li, Jiayao;Guo, Zhanlin;Zhao, Yunlong
对卵黄发生不同阶段的雌性红螯虾（Cheraxquadricarinatus）进行取样，研究可溶性蛋白质、脂质和水含量以及肝胰腺和卵巢的氨基酸和脂肪酸组成的变化。在卵黄发生过程中，肝胰腺和卵巢中可溶性蛋白质和脂质含量的变化取决于饮食和肝胰腺的储备。然而，与卵巢相比，肝胰腺中氨基酸组成的波动表明，从该器官调动的蛋白质资源正在补偿从进食中获得的蛋白质资源。肝胰腺脂肪酸组成的变化对于卵巢中脂质的快速积累显示出有限的代偿功能。两种组织中饱和脂肪酸/单不饱和脂肪酸/多不饱和脂肪酸（PUFA）和主要脂肪酸的比例表明淡水物种中脂肪酸的动员和利用与海洋物种不同。根据红爪的摄食习惯，本研究中使用的商业颗粒可能不是最佳的，含有较少 PUFA 的饮食可能足以满足其卵黄发生并降低饲养成本。
Redclaw crayfish (Cherax quadricarinatus) females at different stages of vitellogenesis were sampled to investigate variations in soluble protein, lipid and water content and in the amino acid and fatty acid composition of the hepatopancreas and ovaries. During vitellogenesis, the changes in the content of soluble proteins and lipids in the hepatopancreas and ovaries were dependent on both diet and the reserves from the hepatopancreas. However, in comparison to the ovary, the fluctuation of the amino acid composition in the hepatopancreas suggested that the protein resources mobilized from this organ was compensating for those gained from feeding. Changes in the fatty acid composition of the hepatopancreas showed limited compensatory function as for the quick accumulation of lipids in the ovaries. The proportional amounts of saturated fatty acids/mono-unsaturated fatty acids/poly-unsaturated fatty acids (PUFA) and the predominant fatty acids in both tissues indicated that the mobilization and utilization of fatty acids in freshwater species are different from that in marine species. Based on the redclaw's feeding habits, the commercial pellets used in this study may not be optimal, and a diet with less PUFA may suffice for its vitellogenesis and reduce the feeding costs.