Physiological, Cytological, and Transcriptomic Analysis of Magnesium Protoporphyrin IX Methyltransferase Mutant Reveal Complex Genetic Regulatory Network Linking Chlorophyll Synthesis and Chloroplast Development in Rice.
Physiological, Cytological, and Transcriptomic Analysis of Magnesium Protoporphyrin IX Methyltransferase Mutant Reveal Complex Genetic Regulatory Network Linking Chlorophyll Synthesis and Chloroplast Development in Rice.
复制标题
DOI：
10.3390/plants12213785
复制
发表时间：
2023-11-06
期刊：
影响因子：
--
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Yao Y;Zhang H;Guo R;Fan J;Liu S;Liao J;Huang Y;Wang Z
叶绿素合成关键基因的功能缺陷通常会导致叶绿体发育异常，但这些关键基因调控叶绿体发育的遗传调控网络尚不清楚。镁原卟啉IX甲基转移酶（ChlM）是叶绿素合成过程中的关键限速酶。生理分析表明，突变体叶绿素和类胡萝卜素含量显著降低。透射电子显微镜观察表明，突变体的叶绿体发育不良，类囊体膜不完整、疏松、不明显。激素含量分析发现，茉莉酸、水杨酸和生长素在突变体中积累。比较转录组分析确定了1534个差异表达基因（DEG）之间的chlm和野生型，其中包括876个上调基因和658个下调基因。基因本体论（GO）和京都基因和基因组百科全书（KEGG）分析表明，这些DEG高度参与叶绿素代谢、叶绿体发育和光合作用。蛋白质-蛋白质相互作用网络分析发现，蛋白质翻译在ChlM基因调控过程中起着至关重要的作用。其中，分别有62个和6个DEG被注释为调节叶绿素和类胡萝卜素代谢; 278个DEG被预测参与调节叶绿体发育; 59个DEG被发现调节激素调节途径; 192个DEG被注释为调节信号途径; 49个DEG被初步鉴定为转录因子。这些基因中的几十个已经被很好地研究，并报道在叶绿素积累或叶绿体发育中发挥重要作用，为所鉴定的DEG的作用的可靠性提供了直接证据。这些结果表明，叶绿素合成和叶绿体发育的ChlM基因的积极调控。这些调控过程涉及到激素、信号通路和转录调控等多个方面。通过定量实时PCR（qRT-PCR）分析验证转录组数据的准确性。本研究揭示了ChlM基因调控叶绿素合成和叶绿体发育的复杂遗传调控网络。对ChlM基因在逆行信号中的作用进行了讨论。茉莉酸、水杨酸或其衍生物在某种未知状态下被认为是叶绿体到细胞核的信号通路中的逆行信号分子。
Functional defects in key genes for chlorophyll synthesis usually cause abnormal chloroplast development, but the genetic regulatory network for these key genes in regulating chloroplast development is still unclear. Magnesium protoporphyrin IX methyltransferase (ChlM) is a key rate-limiting enzyme in the process of chlorophyll synthesis. Physiological analysis showed that the chlorophyll and carotenoid contents were significantly decreased in the chlm mutant. Transmission electron microscopy demonstrated that the chloroplasts of the chlm mutant were not well developed, with poor, loose, and indistinct thylakoid membranes. Hormone content analysis found that jasmonic acid, salicylic acid, and auxin accumulated in the mutant. A comparative transcriptome profiling identified 1534 differentially expressed genes (DEGs) between chlm and the wild type, including 876 up-regulated genes and 658 down-regulated genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that these DEGs were highly involved in chlorophyll metabolism, chloroplast development, and photosynthesis. Protein−protein interaction network analysis found that protein translation played an essential role in the ChlM gene-regulated process. Specifically, 62 and 6 DEGs were annotated to regulate chlorophyll and carotenoid metabolism, respectively; 278 DEGs were predicted to be involved in regulating chloroplast development; 59 DEGs were found to regulate hormone regulatory pathways; 192 DEGs were annotated to regulate signal pathways; and 49 DEGs were putatively identified as transcription factors. Dozens of these genes have been well studied and reported to play essential roles in chlorophyll accumulation or chloroplast development, providing direct evidence for the reliability of the role of the identified DEGs. These findings suggest that chlorophyll synthesis and chloroplast development are actively regulated by the ChlM gene. And it is suggested that hormones, signal pathways, and transcription regulation were all involved in these regulation processes. The accuracy of transcriptome data was validated by quantitative real-time PCR (qRT-PCR) analysis. This study reveals a complex genetic regulatory network of the ChlM gene regulating chlorophyll synthesis and chloroplast development. The ChlM gene’s role in retrograde signaling was discussed. Jasmonic acid, salicylic acid, or their derivatives in a certain unknown state were proposed as retrograde signaling molecules in one of the signaling pathways from the chloroplast to nucleus.
登录
查看更多内容
影响因子：
7.2
作者：
He, Lei;Li, Man;Zhu, Li
通讯作者：
Zhu, Li
GLK1转录因子功能的丧失揭示了叶绿素生物合成和叶绿体发育的新见解
影响因子：
6.9
作者：
Gang, Huixin;Li, Ranhong;Jiang, Jing
通讯作者：
Jiang, Jing
影响因子：
12.3
作者：
Kim D;Pertea G;Trapnell C;Pimentel H;Kelley R;Salzberg SL
通讯作者：
Salzberg SL
水杨酸在维持紫花苜蓿（Medicago sativa L.）对铝毒性耐受性的光合光系统完整性和功能方面的保护作用
影响因子：
6.5
作者：
Cheng, Xiaoqing;Fang, Tingyu;Zhou, Peng
通讯作者：
Zhou, Peng
影响因子：
3.7
作者：
Yang Q;He H;Li H;Tian H;Zhang J;Zhai L;Chen J;Wu H;Yi G;He ZH;Peng X
通讯作者：
Peng X