Infection of hamsters with historical and epidemic BI types of Clostridium difficile
Infection of hamsters with historical and epidemic BI types of Clostridium difficile
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DOI：
10.1086/523106
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发表时间：
2007-12-15
影响因子：
6.4
通讯作者：
Gerding, Dale N.
中科院分区：
文献类型：
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作者：
Razaq, Nadia;Sambol, Susan;Gerding, Dale N.
背景北美和欧洲的医院已经报告了艰难梭菌相关疾病的爆发，其死亡率出乎意料的高，由一组新发现的艰难梭菌引起。艰难梭菌，BI组。我们的目的是比较，在仓鼠，历史上的非流行性BI类型，BI 1，与2个最近流行的BI类型，BI 6和BI 17，并与2个标准的致突变株，K14和630的毒力。对于每种菌株，10只仓鼠给予1剂克林霉素，5天后给予100 ℃。通过胃接种给予的艰难孢子。记录结果。结果。仓鼠模型显示了从接种到死亡的平均时间的变化（对于BI 6，40 h;对于BI 1，48 h;对于K14，49 h;对于BI 17，69 h;对于630，102 h;对于BI 6、BI 1和K14 vs. 630，P < .01;对于BI 17 vs. 630，P < .05）和从定殖到死亡（对于BI 1，7 h;对于BI 17，13 h;对于BI 6，16 h;对于K14，17 h;对于630，52 h;对于BI 1、BI 17、BI 6和K14 vs. 630，P < .01）。BI组菌株的致死性并不比标准毒素型0菌株K14更快，但比标准毒素型0菌株630更快。BI 6是我们收集的最常见的BI类型，在仓鼠中毒性特别强，在接种后48小时内持续导致死亡。
Background. North American and European hospitals have reported outbreaks of Clostridium difficile-associated disease with unexpectedly high mortality caused by a newly recognized group of C. difficile strains, group BI. Our objective was to compare, in hamsters, the virulence of a historical nonepidemic BI type, BI1, with that of 2 recent epidemic BI types, BI6 and BI17, and with that of 2 standard toxigenic strains, K14 and 630.Methods. For each strain, 10 hamsters were given 1 dose of clindamycin, followed 5 days later with 100 C. difficile spores administered by gastric inoculation. Outcomes were recorded.Results. The hamster model demonstrated variations in mean times from inoculation to death (for BI6, 40 h; for BI1, 48 h; for K14, 49 h; for BI17, 69 h; for 630, 102 h; for BI6, BI1, and K14 vs. 630, P < .01; for BI17 vs. 630, P < .05) and from colonization to death (for BI1, 7 h; for BI17, 13 h; for BI6, 16 h; for K14, 17 h; for 630, 52 h; for BI1, BI17, BI6, and K14 vs. 630, P < .01).Conclusion. Group BI strains were not more rapidly fatal than the standard toxinotype 0 strain K14 but were more rapidly fatal than the standard toxinotype 0 strain 630. BI6, the most common BI type in our collection, was particularly virulent in hamsters, consistently causing death within 48 h of inoculation.