Inhibition of SAH-hydrolase activity during seed germination leads to deregulation of flowering genes and altered flower morphology in tobacco
Inhibition of SAH-hydrolase activity during seed germination leads to deregulation of flowering genes and altered flower morphology in tobacco
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DOI：
10.1007/s00438-011-0601-8
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发表时间：
2011-03-01
影响因子：
3.1
通讯作者：
Kovarik, Ales
中科院分区：
文献类型：
--
作者：
Fulnecek, Jaroslav;Matyasek, Roman;Kovarik, Ales
发育过程与表观遗传信息的某些状态密切相关，其中依赖于染色质的甲基化。s -腺苷蛋氨酸（SAM）和s -腺苷同型半胱氨酸（SAH）是催化DNA和组蛋白甲基化的关键辅因子。为了研究SAH/SAM水平改变对植物发育的影响，我们在萌发期（6天）短时间内将SAH水解酶抑制剂9-(S)-（2,3-二羟丙基）-腺嘌呤（DHPA）施用于烟草种子。瞬时药物处理诱导：(1)剂量依赖性整体DNA低甲基化有丝分裂传递给成体植物；(2)多效性发育缺陷，包括顶端优势度下降、叶花对称性改变、花轮畸形和育性降低；(3)花器官识别基因NTDEF、NTGLO和NAG1在叶片中显著上调。我们得出结论，sah -水解酶抑制可能通过染色质甲基化改变来解除花基因的表达。这些数据进一步表明，在关键发育时期，植物可能对SAH/SAM水平的准确设置特别敏感。
Developmental processes are closely connected to certain states of epigenetic information which, among others, rely on methylation of chromatin. S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) are key cofactors of enzymes catalyzing DNA and histone methylation. To study the consequences of altered SAH/SAM levels on plant development we applied 9-(S)-(2,3-dihydroxypropyl)-adenine (DHPA), an inhibitor of SAH-hydrolase, on tobacco seeds during a short phase of germination period (6 days). The transient drug treatment induced: (1) dosage-dependent global DNA hypomethylation mitotically transmitted to adult plants; (2) pleiotropic developmental defects including decreased apical dominance, altered leaf and flower symmetry, flower whorl malformations and reduced fertility; (3) dramatic upregulation of floral organ identity genes NTDEF, NTGLO and NAG1 in leaves. We conclude that temporal SAH-hydrolase inhibition deregulated floral genes expression probably via chromatin methylation changes. The data further show that plants might be particularly sensitive to accurate setting of SAH/SAM levels during critical developmental periods.