Claudin-6, 7, or 9 Overexpression in the Human Gastric Adenocarcinoma Cell Line AGS Increases Its Invasiveness, Migration, and Proliferation Rate
Claudin-6, 7, or 9 Overexpression in the Human Gastric Adenocarcinoma Cell Line AGS Increases Its Invasiveness, Migration, and Proliferation Rate
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DOI：
10.3109/07357907.2010.512594
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发表时间：
2011-01-01
影响因子：
2.4
通讯作者：
Patricia Rendon-Huerta, Erika
中科院分区：
文献类型：
--
作者：
Elizabeth Zavala-Zendejas, Veronica;Cristina Torres-Martinez, Ana;Patricia Rendon-Huerta, Erika
Claudin表达改变与转移潜能、预后不良或肿瘤复发有关。我们分析了在AGS细胞中claudin-6、claudin-7或claudin-9的过表达是否改变了细胞的运动性、侵袭性或增殖率。克拉丁-7、克拉丁-9和克拉丁-6的侵袭能力分别提高了3.4倍、1.6倍和2.0倍。Claudin-6和claudin-9可促进细胞迁移，而claudin-6、claudin-7和claudin-9转染组细胞增殖率分别增加12.7%、9.0%和13.3%。Claudin-7和claudin-9的过表达增加了claudin-1和阻塞性小带-1的水平。综上所述，claudin-6、claudin-7或claudin-9的个体表达增加足以增强胃腺癌细胞系的致瘤特性。
Altered claudin expression is related to metastatic potential, poor prognosis, or tumor recurrence. We analyzed if the overexpression of claudin-6, claudin-7, or claudin-9 in AGS cells altered cell motility, invasiveness, or proliferation rate. Claudin-7, claudin-9, and claudin-6 enhanced their invasive potential by 3.4-fold, 1.6-fold, and 2.0-fold, respectively. Claudin-6 and claudin-9 enhanced cell migration, while the proliferation rate of claudin-6-, claudin-7-, and claudin-9-transfected cells increased by 12.7%, 9.0%, and 13.3%, respectively. Claudin-7 and claudin-9 overexpression increased claudin-1 and zonula occludens-1 levels. In summary, individual increased expression of claudin-6, claudin-7, or claudin-9 is sufficient to enhance tumorigenic properties of a gastric adenocarcinoma cell line.