Identification and characterization of simple sequence repeat (SSR) markers derived in silico from Brassica oleracea genome shotgun sequences
Identification and characterization of simple sequence repeat (SSR) markers derived in silico from Brassica oleracea genome shotgun sequences
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DOI：
10.1111/j.1471-8286.2006.01488.x
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发表时间：
2006-12-01
期刊：
影响因子：
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通讯作者：
Batley, Jacqueline
中科院分区：
文献类型：
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作者：
Burgess, Benjamin;Mountford, Hayley;Batley, Jacqueline
利用霰弹枪测序程序获得的序列数据，可以挖掘简单序列重复序列（SSRs），为作物改良提供有用的遗传标记。本研究从甘蓝（Brassica oleracea）霰弹枪序列中获得了40个SSR标记，并进行了标记间的交叉扩增。这些标记具有可靠的扩增、基因组特异性和显著的多态性，证明了ssr在商业芸苔种质资源遗传分析中的应用价值。
The availability of sequence data derived from shotgun sequencing programs enables mining for simple sequence repeats (SSRs), providing useful genetic markers for crop improvement. This study presents the development and characterization of 40 SSR markers from Brassica oleracea shotgun sequence and their cross-amplification across Brassica species. The markers show reliable amplification, genome specificity and considerable polymorphism, demonstrating the utility of SSRs for genetic analysis of commercial Brassica germplasm.