Anti-cholinesterases and memory improving effects of Vietnamese Xylia xylocarpa.
Anti-cholinesterases and memory improving effects of Vietnamese Xylia xylocarpa.
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DOI：
10.1186/s13065-016-0197-5
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发表时间：
2016
影响因子：
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通讯作者：
Van Can M
中科院分区：
文献类型：
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作者：
Lam LM;Nguyen MT;Nguyen HX;Dang PH;Nguyen NT;Tran HM;Nguyen HT;Nguyen NM;Min BS;Kim JA;Choi JS;Van Can M
阿尔茨海默病（AD）是老年人痴呆症的最常见原因，其特征在于记忆和其他认知功能的丧失。β-淀粉样蛋白斑块和神经纤维缠结周围的AChE（胆碱能神经系统中的关键酶）水平升高是AD神经病理学的常见特征。东莨菪碱（乙酰胆碱受体拮抗剂）的药理作用可以在各种行为测试中研究，如Morris水迷宫、物体识别、Y-迷宫和被动回避。在本文的范围内，我们报告的抗乙酰胆碱酯酶，抗BChE特性的分离的化合物和木果木霉（MEXX）的甲醇提取物对东莨菪碱诱导的记忆缺陷的体内作用。在进一步的植物化学研究中，分离到一个新的Hopan型三萜类化合物，（3β）-Hopan-3-ol-28，22-dichloro（1），以及20个已知化合物（2-21）。化合物1、2、4、5、7-9和11-13以浓度依赖性方式表现出有效的乙酰胆碱酯酶（AChE）抑制活性，IC 50值范围为54.4 - 94.6 μM。化合物13还显示出抗丁酰胆碱酯酶（BChE）活性，IC 50值为42.7 μM。进行Morris水迷宫、Y-迷宫和物体识别实验。值得注意的是，MEXX口服给药小鼠时有效，实验结果与该药用植物物种的传统用途一致。本文的在线版本（doi：10.1186/s13065-016-0197-5）包含补充材料，可供授权用户使用。
Alzheimer’s disease (AD) is the most common cause of dementia among the elderly and is characterized by loss of memory and other cognitive functions. An increase in AChE (a key enzyme in the cholinergic nervous system) levels around β-amyloid plaques and neurofibrillary tangles is a common feature of AD neuropathology. Amnesic effects of scopolamine (acetylcholine receptor antagonist) can be investigated in various behavioral tests such as Morris water maze, object recognition, Y-maze, and passive avoidance. In the scope of this paper, we report the anti-AChE, anti-BChE properties of the isolated compound and the in vivo effects of the methanolic extract of Xylia xylocarpa (MEXX) on scopolamine-induced memory deficit. In further phytochemistry study, a new hopan-type triterpenoid, (3β)-hopan-3-ol-28,22-olide (1), together with twenty known compounds were isolated (2–21). Compound 1, 2, 4, 5, 7–9, and 11–13 exhibited potent acetylcholinesterase (AChE) inhibitory activity in a concentration-dependent manner with IC50 values ranging from 54.4 to 94.6 μM. Compound 13 was also shown anti-butyrylcholinesterase (BChE) activity with an IC50 value of 42.7 μM. The Morris water Y-maze, Y-maze, and object recognition test were also carried out. It is noteworthy that MEXX is effective when administered orally to mice, experimental results are consistent with the traditional use of this medicinal plant species. The online version of this article (doi:10.1186/s13065-016-0197-5) contains supplementary material, which is available to authorized users.