Efficient reactivation of latent herpes simplex virus from mouse central nervous system tissues
Efficient reactivation of latent herpes simplex virus from mouse central nervous system tissues
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DOI：
10.1128/jvi.01232-06
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发表时间：
2006-12-01
影响因子：
5.4
通讯作者：
Chen, Shun-Hua
中科院分区：
文献类型：
--
作者：
Chen, Shih-Heng;Yao, Hui-Wen;Chen, Shun-Hua
几十年来，许多离体研究已经证明，潜伏的单纯疱疹病毒（HSV）有效地从神经节，但很少从中枢神经系统（CNS），当通过在外植体培养前切碎组织进行测定时，尽管病毒基因组存在于两个网站。在这里，我们表明，88%的小鼠脑干重新激活潜伏病毒时，他们被解离成细胞悬浮液前离体外植体培养。用一种以上的病毒株、病毒血清型和小鼠株证明了HSV从小鼠CNS的有效再活化，进一步表明CNS可能是HSV的真实潜伏位点，具有引起复发性疾病的潜力。
For decades, numerous ex vivo studies have documented that latent herpes simplex virus (HSV) reactivates efficiently from ganglia, but rarely from the central nervous systems (CNS), of mice when assayed by mincing tissues before explant culture, despite the presence of viral genomes in both sites. Here we show that 88% of mouse brain stems reactivated latent virus when they were dissociated into cell suspensions before ex vivo explant culture. The efficient reactivation of HSV from the mouse CNS was demonstrated with more than one viral strain, viral serotype, and mouse strain, further indicating that the CNS can be an authentic latency site for HSV with the potential to cause recurrent disease.