Quantification of structural properties of cell-sized individual liposomes by flow cytometry
Quantification of structural properties of cell-sized individual liposomes by flow cytometry
复制标题
DOI：
10.1263/jbb.102.171
复制
发表时间：
2006-09-01
影响因子：
2.8
通讯作者：
Yomo, Tetsuya
中科院分区：
文献类型：
--
作者：
Sato, Kanetomo;Obinata, Kei;Yomo, Tetsuya
We describe a new high-throughput method of quantifying the structural properties of individual cell-sized liposomes. An internal aqueous solution of liposomes was labeled with a green fluorescent marker and the membrane with a red marker. The double-labeled liposomes were analyzed using flow cytometry, and the internal aqueous volume and lipid membrane volume of each liposome were measured. The experimental results indicate that both the internal aqueous and lipid membrane volumes positively correlate with the intensity of forward-scatter (ITS) and side-scatter (SS) signals in a logarithmic scale. In addition, liposomes in 18 small areas gated by log(FS) and log(SS) were sorted by fluorescence-activated cell sorting (FACS), and observed by optical microscopy. Structural characteristics observed in the microscopy images of heterogeneous liposomes correlated with FACS data. Because this method does not employ any particular assumption about the shape and structure of liposomes, flow cytometry is a powerful tool for estimating the internal and membrane volumes of individual cell-sized liposomes with heterogeneous shapes and structures.
