Realistic Numerical and Analytical Modeling of Light Scattering in Brain Tissue for Optogenetic Applications(1,2,3).
Realistic Numerical and Analytical Modeling of Light Scattering in Brain Tissue for Optogenetic Applications(1,2,3).
复制标题
DOI：
10.1523/eneuro.0059-15.2015
复制
发表时间：
2016-01
期刊：
影响因子：
3.4
通讯作者：
Shoham S
中科院分区：
文献类型：
--
作者：
Yona G;Meitav N;Kahn I;Shoham S
近年来，光遗传学已成为神经科学研究的核心工具。估计可见光通过脑组织的传输对于控制不同深度神经元的激活水平、设计光学系统以及避免功率密度过高造成的损伤至关重要。库贝尔卡-蒙克模型和蒙特卡罗模拟以前曾被用来模拟光通过啮齿类动物脑组织的传播，然而，这些先前的尝试都存在根本性的缺陷。在这里，我们介绍并研究了两种修改方法，用于对多模光纤发出的光分布和组织散射进行建模，使用真实的数值蒙特卡罗模拟和基于光束扩展函数方法的分析方法。我们证明了新方法的预测与最近发布的数据以及小鼠大脑皮层切片的新测量结果非常一致，我们的结果在 λ = 473 nm 处产生了~47 µm 的新皮层散射长度估计，明显短于光遗传学应用中通常假设的值。
In recent years, optogenetics has become a central tool in neuroscience research. Estimating the transmission of visible light through brain tissue is of crucial importance for controlling the activation levels of neurons in different depths, designing optical systems, and avoiding lesions from excessive power density. The Kubelka–Munk model and Monte Carlo simulations have previously been used to model light propagation through rodents' brain tissue, however, these prior attempts suffer from fundamental shortcomings. Here, we introduce and study two modified approaches for modeling the distributions of light emanating from a multimode fiber and scattering through tissue, using both realistic numerical Monte Carlo simulations and an analytical approach based on the beam-spread function approach. We demonstrate a good agreement of the new methods' predictions both with recently published data, and with new measurements in mouse brain cortical slices, where our results yield a new cortical scattering length estimate of ∼47 µm at λ = 473 nm, significantly shorter than ordinarily assumed in optogenetic applications.