A Monoclonal Antibody Against Transmissible Gastroenteritis Virus Generated via Immunization of a DNA Plasmid Bearing TGEV S1 Gene
A Monoclonal Antibody Against Transmissible Gastroenteritis Virus Generated via Immunization of a DNA Plasmid Bearing TGEV S1 Gene
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通过免疫带有 TGEV S1 基因的 DNA 质粒产生的抗传染性胃肠炎病毒的单克隆抗体
DOI：
10.1089/mab.2012.0067
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发表时间：
2013-02-01
影响因子：
--
通讯作者：
Ren, Xiaofeng
中科院分区：
文献类型：
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作者：
Zhao, Qiong;Zhu, Jiayi;Ren, Xiaofeng
传染性胃肠炎病毒（TGEV）是冠状病毒的一员。 TGEV 的病毒刺突 (S) 蛋白介导 TGEV 与其易感细胞之间的相互作用。在此，使用带有TGEV S1基因（TGEV S基因的N末端一半）的DNA质粒免疫BALB/c小鼠，然后使用杂交瘤技术产生单克隆抗体（MAb）。生成的 MAb (1H4) 通过 ELISA 进行鉴定。免疫荧光测定表明 MAb 1H4 能够检测 TGEV 细胞的感染。 MAb 1H4 将 TGEV 与其他对照病毒区分开来。此外，虽然MAb 1H4的类型是IgM，但它可以以剂量依赖的方式减少TGEV对细胞的感染。
Transmissible gastroenteritis virus (TGEV) is a member of the coronaviruses. The viral spike (S) protein of TGEV mediates interaction between TGEV and its susceptible cells. Herein, DNA plasmid bearing TGEV S1 gene (the N terminal half of TGEV S gene) was used to immunize BALB/c mice followed by generation of a monoclonal antibody (MAb) using the hybridoma technique. The generated MAb (1H4) was identified by ELISA. Immunofluorescence assays showed that MAb 1H4 was able to detect infection of cells with TGEV. The MAb 1H4 distinguished TGEV from other control viruses. Additionally, although the type of MAb 1H4 was IgM, it could reduce cell infection by TGEV in a dose-dependent manner.