Awareness and knowledge of allergens: A need and a challenge to assure a safe and healthy consumption of small fruits
Awareness and knowledge of allergens: A need and a challenge to assure a safe and healthy consumption of small fruits
复制标题
对过敏原的认识和知识：确保安全健康消费小水果的需求和挑战
DOI：
10.3233/br-2010-012
复制
发表时间：
2010
影响因子：
1.7
通讯作者：
F. Maghuly
中科院分区：
文献类型：
--
作者：
M. Laimer;F. Maghuly
食物过敏是一种普遍的现象：估计有1-2%的人口患有某种类型的食物过敏。水果和蔬菜被认为是食物过敏的最重要的诱发物之一。浆果类水果富含黄酮醇、黄酮和花青素等酚类化合物，对人体健康可能具有有益的作用，近年来引起了人们越来越多的关注。然而，它们可能含有一系列致敏蛋白，导致不适，甚至对某些人构成严重威胁。水果中过敏原的鉴定和表征，如来自遥远类群的浆果，需要涉及基因组和蛋白质组学工具的新方法。测定了蓝莓（欧洲越橘）、草莓（草莓）、树莓（树莓）和黑莓（树莓）的过敏原含量。不同的提取程序，需要提高蛋白质检测与多克隆和单克隆抗血清提出了不同的过敏原从苹果在Western印迹。由于交叉反应性，它们能够识别其他植物物种的高度保守蛋白质家族中的相似表位。果实提取物进行了分析，通过二维电泳和质谱。使用来自大量患者和患者血清的临床筛选工具，对小水果中存在的过敏蛋白质提供了结论性结果。
Food allergy is a widespread phenomenon: An estimated 1–2% of the population suffers from some type of food allergy. Fruits and vegetables are considered among the most important elicitors of food allergy. Berry fruits rich in phenolic compounds including flavonols, flavones and anthocyanins have recently gained increasing interest due to their possible beneficial effects on human health. However, they may harbour a series of allergenic proteins that cause discomfort or even represent serious threats to certain individuals. The identification and characterization of allergens in fruits like berries from distant taxa, requires novel approaches involving genomic and proteomic tools. The allergen content of blueberries (Vaccinium myrtillus), strawberries (Fragaria ananassa), raspberries (Rubus idaeus), and blackberries (Rubus fruticosus) was determined. Different extraction procedures are required to improve protein detection with polyclonal and monoclonal antisera raised against different allergens from Malus domestica in Western blotting. Due to the cross-reactivity they are able to recognize similar epitopes in highly conserved protein families of other plant species. Fruit extracts were analysed by two dimensional electrophoresis and mass spectrometry. Using clinical screening tools from a high number of patients and patient sera delivered conclusively results on allergenic proteins present in the small fruits.