NERVE GROWTH-FACTOR COUNTERACTS THE NEUROPHYSIOLOGICAL AND NEUROCHEMICAL EFFECTS OF CHRONIC SCIATIC-NERVE SECTION
NERVE GROWTH-FACTOR COUNTERACTS THE NEUROPHYSIOLOGICAL AND NEUROCHEMICAL EFFECTS OF CHRONIC SCIATIC-NERVE SECTION
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DOI：
10.1016/0006-8993(85)90396-8
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发表时间：
1985-01-01
期刊：
影响因子：
2.9
通讯作者：
EMSON, PC
中科院分区：
文献类型：
--
作者：
FITZGERALD, M;WALL, PD;EMSON, PC
将大鼠坐骨神经单侧切断，并使用留置渗透泵将神经生长因子（NGF）局部应用于神经残端，持续10-14天。神经生长因子是否有任何影响以前报道的神经生理和神经化学事件发生中枢周围神经病变进行了测试。应用方法允许将另一侧的坐骨神经用作对照。初级传入去极化下降，如预期的那样，慢性神经节后，其控制值的13%，但如果神经生长因子的管理，它下降到只有43.5%的控制。慢性神经切断也被认为会导致去传入背角细胞的感受野扩大。神经生长因子治疗减少了50%的数量这样大的感受野。在神经生长因子治疗后，背角切断的神经末梢中抗氟酸性磷酸酶的正常消耗没有发生。P物质的放射免疫分析表明，30%的减少后角的水平，慢性坐骨神经节没有发生时，应用神经生长因子，并伴随着60%的减少，背根神经节的水平被改变为64%的增加神经生长因子。对切断的坐骨神经进行慢性神经生长因子治疗确实部分逆转了正常情况下传入神经切断后的中枢变化。
The sciatic nerve was sectioned unilaterally in rats and nerve growth factor (NGF) applied locally to the nerve stump for the following 10-14 days using an indwelling osmotic pump. Whether NGF had any effect on the previously reported neurophysiological and neurochemical events that occur central to a peripheral nerve lesion was tested. The method of application allowed the sciatic nerve on the other side to be used as a control. Primary afferent depolarization fell, as expected, to 13% of its control value after chronic nerve section but if NGF was administered it fell to only 43.5% of control. Chronic nerve section is also known to result in expansion of the receptive fields of deafferented dorsal horn cells. NGF treatment reduced the number of such large receptive fields by 50%. The normal depletion of fluoride resistant resistant acid phosphatase from the cut nerve terminals in the dorsal horn did not occur following NGF treatment. Radioimmunoassay of substance P revealed that the 30% reduction in dorsal horn levels that follows chronic sciatic nerve section did not occur when NGF was applied and that the accompanying 60% decrease in dorsal root ganglion levels was changed to a 64% increase by NGF. Chronic NGF treatment of a cut sciatic nerve does partially reverse the central changes that normally follow deafferentation.