Effect of dietary ethanol and cholesterol on phospholipid composition of hepatic mitochondria and microsomes from the monkey, Macaca nemestrina.
Effect of dietary ethanol and cholesterol on phospholipid composition of hepatic mitochondria and microsomes from the monkey, Macaca nemestrina.
复制标题
膳食乙醇和胆固醇对猕猴肝线粒体和微粒体磷脂组成的影响。
DOI：
10.1111/j.1530-0277.1981.tb04925.x
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Leathers,C
中科院分区：
文献类型：
--
作者：
Cunningham,CC;Sinthusek,G;Spach,PI;Leathers,C
猕猴(Macaca Nomestrina)被分成四组，每组都得到了特定的饮食。饮食中的变量如下：饮食A，0.3毫克胆固醇/千卡营养素；饮食B，1.0毫克胆固醇/千卡营养素；饮食C，0.3毫克胆固醇/千卡营养素，乙醇(热量的36%)；饮食D，1.0毫克胆固醇/千卡营养素，乙醇(热量的36%)。饮食中含有乙醇的猴子会患上脂肪肝。从这些肝脏分离的线粒体和微粒体表现出乙醇引起的代谢功能的变化，如前文所述。1伴随着代谢活性的变化的是细胞器磷脂的变化，这种变化同时受到饮食中乙醇和胆固醇的影响。可归因于乙醇的变化如下。微粒体中磷脂酰乙醇胺含量减少，线粒体中磷脂酰乙醇胺含量增加，微粒体中鞘磷脂含量显著增加。线粒体和微粒体磷脂中硬脂酸和花生四烯酸含量升高，棕榈酸和油酸含量降低。胆固醇影响了几种磷脂的脂肪酸组成，通常与那些归因于乙醇的变化方向相反。胆固醇喂养增加了棕榈酸和油酸的水平，减少了几种磷脂中硬脂酸、亚油酸和花生四烯酸的含量。本研究中观察到的乙醇和胆固醇引起的显著变化表明，线粒体和微生物体代谢功能的变化至少部分地受这些有机微体的磷脂组成的变化控制，至少部分地受这些细胞器的磷脂组成的变化控制。
Monkeys(Macaca nemestrina)were divided into four groups, and each group was fed a particular diet. The variables in the diets were as follows: diet A, 0.3 mg cholesterol/kcal nutrient; diet B, 1.0 mg cholesterol/kcal nutrient; diet C, 0.3 mg cholesterol/kcal nutrient, ethanol (36% of calories); diet D, 1.0 mg cholesterol/ kcal nutrient, ethanol (36% of calories). Monkeys on the diets containing ethanol developed fatty liver. Mitochondria and microsomes isolated from these livers demonstrated ethanol‐elicited alterations in metabolic functions as is described in the preceding paper.1Accompanying these changes in metabolic activities were alterations in organelle phospholipids that were influenced by both dietary ethanol and cholesterol. The changes that could be attributed to ethanol were as follows. Phosphatidyl ethanolamine was decreased in microsomes and increased in mitochondria; the sphingomyelin content in microsomes was increased significantly. The levels of stearic and arachidonic acid were elevated, and palmitic and oleic acid decreased, in phospholipids from both mitochondria and microsomes. Cholesterol influenced the fatty acid composition of several phospholipids, usually in a direction opposite to those alterations attributed to ethanol. Cholesterol feeding increased levels of palmitic and oleic acid and decreased amounts of stearic, linoleic, and arachidonic acid in several phospholipids. The significant ethanol‐ and cholesterol‐elicited alterations observed in this study suggest the possibility that the changes in metabolic functions in mitochondria and microsomes are controlled, at least in part, by alterations in the phospholipid compositions of these organmicrosomes are controlled, at least In part, by alterations in the phospholipid compositions of these organelles.