Humoral immune responses to Plasmodium fakiparum among HIV-1-infected Kenyan adults
Humoral immune responses to Plasmodium fakiparum among HIV-1-infected Kenyan adults
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DOI：
10.1002/prca.201100021
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发表时间：
2011-12-01
影响因子：
2
通讯作者：
Vigil, Adam
中科院分区：
文献类型：
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作者：
Nnedu, Obinna N.;O'Leary, Michael P.;Vigil, Adam
简介：体液免疫反应在疟疾自然获得性免疫力中发挥着关键作用。了解疟疾风险较高的个体中哪些体液反应受损，可能会提高我们对疟疾免疫控制的了解，并有助于疫苗的开发。方法：我们比较了基苏木（高、长年疟疾传播导致部分免疫）成年人和基什（低、季节性疟疾传播）成年人之间 483 种恶性疟原虫抗原的体液反应。然后在每个站点内，我们比较了疟疾高危人群 (CD4+ 500) 之间的疟疾特异性体液反应。使用含有 483 个恶性疟原虫抗原和 71 个 HIV 抗原的蛋白质微阵列芯片。进行 Ben jamini-Hochberg 调整以控制多重比较。结果：包括 CSP、MSP1、LSA1 和 AMA1 在内的 57 种抗原被确定在 Kisumu 中比 Kisii 中的反应性显着更高。在早期的研究中，其中十种抗原已被鉴定为具有保护作用。 CD4+ T 细胞计数对体液反应没有显着影响。结论：蛋白质微阵列是同时筛选多种体液反应的有用方法。这项研究为潜在的候选疫苗提供了有用的线索。 CD4 计数的适度减少可能不会显着影响疟疾特异性体液免疫。
Introduction: Humoral immune responses play a pivotal role in naturally acquired immunity to malaria. Understanding which humoral responses are impaired among individuals at higher risk for malaria may improve our understanding of malaria immune control and contribute to vaccine development.Methods: We compared humoral responses with 483 Plasmodium falciparum antigens between adults in, Kisumu (high, year-long malaria transmission leading to partial immunity), and adults in Kish (low, seasonal malaria transmission). Then within each site, we compared malaria-specific humoral responses between those at higher risk for malaria (CD4+ 500). A protein microarray chip containing 483 P. falciparum antigens and 71 HIV antigens was used. Ben jamini-Hochberg adjustments were made to control for multiple comparisons.Results: Fifty-seven antigens including CSP, MSP1, LSA1 and AMA1 were identified as significantly more reactive in Kisumu than in Kisii. Ten of these antigens had been identified as protective in an earlier study. CD4+ T-cell count did not significantly impact humoral responses.Conclusion: Protein microarrays are a useful method to screen multiple humoral responses simultaneously. This study provides useful clues for potential vaccine candidates. Modest decreases in CD4 counts may not significantly impact malaria-specific humoral immunity.