Molecular detection of Histoplasma capsulatum var. capsulatum in human clinical samples
Molecular detection of Histoplasma capsulatum var. capsulatum in human clinical samples
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DOI：
10.1128/jcm.41.4.1753-1755.2003
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发表时间：
2003-04-01
影响因子：
9.4
通讯作者：
Serra, E
中科院分区：
文献类型：
--
作者：
Bracca, A;Tosello, ME;Serra, E
我们开发了一种用于组织浆菌病诊断的半植入式PCR，该PCR扩增了部分荚膜组织浆菌H抗原基因。该分析具有高度的敏感性和特异性，能够检测到少于10个酵母细胞对应的基因组物质，而不会与其他细菌或真菌病原体发生交叉反应。
We developed a seminested PCR for the diagnosis of histoplasmosis that amplifies a portion of the Histoplasma capsulatum H antigen gene. This assay is highly sensitive and specific, being able to detect genomic material corresponding to less than 10 yeast cells without cross-reaction against other bacterial or fungal pathogens.