Apocarotenoids determination in Capsicum chinense Jacq. cv. Habanero, by supercritical fluid chromatography-triple-quadrupole/mass spectrometry
Apocarotenoids determination in Capsicum chinense Jacq. cv. Habanero, by supercritical fluid chromatography-triple-quadrupole/mass spectrometry
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DOI：
10.1016/j.foodchem.2017.03.145
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发表时间：
2017-09-15
期刊：
影响因子：
8.8
通讯作者：
Mondello, Luigi
中科院分区：
文献类型：
--
作者：
Giuffrida, Daniele;Zoccali, Mariosimone;Mondello, Luigi
目前，食品中类胡萝卜素的液相色谱分离研究相对较少，而采用SFC-APCI（+/-）/QqQ/MS分离类胡萝卜素的研究更是空白。本研究的双重目的是研究辣椒中类胡萝卜素的含量，并建立一种新的SFC-APCI（+/-）/QqQ/MS方法，本研究首次采用新型的C30柱对脱辅基类胡萝卜素进行了快速、高效的检测，共鉴定出25种脱辅基类胡萝卜素，其中14种为游离脱辅基类胡萝卜素，11种为脱辅基类胡萝卜素脂肪酸酯。该方法允许在不到五分钟的时间内进行所有分离。检测到的Apo-10 '-、Apo-14'-和Apo-15-辣椒红醛以及不同的Apo-8 '-辣椒红醛和Apo-100-玉米黄嘌呤脂肪酸酯先前尚未在任何辣椒属物种中以及据我们所知在任何食品基质中初步鉴定。(C)2017爱思唯尔有限公司版权所有。
Relatively few studies are available in the literature on the apocarotenoids occurrence in food which are based on liquid chromatographic separation approaches and no reports are available on the apocarotenoids separation by SFC-APCI (+/-)/QqQ/MS. The dual purpose of this research was to investigate the apocarotenoids presence in red habanero peppers and to develop a novel SFC-APCI(+/-)/QqQ/MS methodology, using a novel fused-core C30 column, for the apocarotenoids detection in a fast and efficient way, for the first time.In this study, 25 different apocarotenoids were identified, 14 were free apocarotenoids and 11 were apocarotenoids fatty acids esters. The methodology allowed for all the separations to occur in less then five minutes. The detected Apo-10'-, Apo-14'-and Apo-15-capsorubinals and different Apo-8'-capsorubinal and Apo-100-zeaxanthinals fatty acid esters had not been previously tentatively identified in any Capsicum species and, to the best of our knowledge, in any food matrix. (C) 2017 Elsevier Ltd. All rights reserved.