Transcriptome profiling reveals roles of meristem regulators and polarity genes during fruit trichome development in cucumber (Cucumis sativus L.)
Transcriptome profiling reveals roles of meristem regulators and polarity genes during fruit trichome development in cucumber (Cucumis sativus L.)
复制标题
转录组分析揭示了分生组织调节因子和极性基因在黄瓜果实毛状体发育过程中的作用（Cucumis sativus L.）
DOI：
10.1093/jxb/eru258
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发表时间：
2014-09-01
影响因子：
6.9
通讯作者：
Zhang, Xiaolan
中科院分区：
文献类型：
--
作者：
Chen, Chunhua;Liu, Meiling;Zhang, Xiaolan
毛状体是表皮毛发状结构，在植物防御生物和非生物胁迫中发挥作用。人们对拟南芥和番茄的叶毛发育进行了广泛的研究，但果实毛状体形成的分子机制仍然难以捉摸。黄瓜果实上布满毛状体（刺），直接影响黄瓜产品的外观和品质。在这里，我们表征了野生型（WT）黄瓜和自发突变体微小分枝毛（tbh）的果实脊柱发育。我们的数据表明，黄瓜毛状体是多细胞且非腺体的，具有畸形的细胞器并且没有核内复制。果实脊柱发育通常是均匀的，并以快速基部扩张阶段为特征。 tbh突变体中的毛状体很小且分枝，密度增加且细胞形状异常。转录组分析表明，分生组织相关基因在 tbh 与 WT 中的上调基因中高度富集，以及在碱基扩展之后与之前的 WT 脊柱中高度富集，并且在脊柱碱基扩展期间极大地诱导了极性调节因子。定量逆转录PCR和原位杂交证实了CUP-SHAPED COTYLEDON3 (CUC3)和SHOOT MERISTEMLESS (STM)在脊柱发育过程中的差异表达。因此，黄瓜毛状体在形态上与拟南芥和番茄的毛状体不同，它们的发育可能受到涉及分生组织基因和极性调节因子的独特途径的调节。
Trichomes are epidermal hair-like structures that function in plant defence against biotic and abiotic stresses. Extensive studies have been performed on foliar trichomes development in Arabidopsis and tomato, but the molecular mechanism of fruit trichome formation remains elusive. Cucumber fruit is covered with trichomes (spines) that directly affect the appearance and quality of cucumber products. Here, we characterized the fruit spine development in wild-type (WT) cucumber and a spontaneous mutant, tiny branched hair (tbh). Our data showed that the cucumber trichome was multicellular and non-glandular, with malformed organelles and no endoreduplication. Fruit spine development was generally homogenous and marked by a rapid base expansion stage. Trichomes in the tbh mutant were tiny and branched, with increased density and aberrant cell shape. Transcriptome profiling indicated that meristem-related genes were highly enriched in the upregulated genes in the tbh versus the WT, as well as in WT spines after versus before base expansion, and that polarity regulators were greatly induced during spine base expansion. Quantitative reverse transcription PCR and in situ hybridization confirmed the differential expression of CUP-SHAPED COTYLEDON3 (CUC3) and SHOOT MERISTEMLESS (STM) during spine development. Therefore, cucumber trichomes are morphologically different from those of Arabidopsis and tomato, and their development may be regulated by a distinct pathway involving meristem genes and polarity regulators.