Targeted toxicants to dopaminergic neuronal cell death.
Targeted toxicants to dopaminergic neuronal cell death.
复制标题
DOI：
10.1007/978-1-4939-2152-2_18
复制
发表时间：
2015
影响因子：
--
通讯作者：
Huajun Jin;A. Kanthasamy;Dilshan S. Harischandra;V. Anantharam;A. Rana;A. Kanthasamy
中科院分区：
文献类型：
--
作者：
Huajun Jin;A. Kanthasamy;Dilshan S. Harischandra;V. Anantharam;A. Rana;A. Kanthasamy
Parkinson’s disease (PD ) is mainly characterized by a progressive degeneration of dopaminergic neurons in the substantia nigra resulting in chronic deficits in motor functions. Administration of the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP ) produces PD symptoms and recapitulates the main features of PD in human and animal models . MPTP is converted to 1-methyl-4-phenylpyridine (MPP+), which is the active toxic compound that selectively destroys dopaminergic neurons. Here, we describe methods and protocols to evaluate MPTP/MPP+-induced dopaminergic neurodegeneration in both murine primary mesencephalic cultures and animal models. The ability of MPTP/MPP+to cause dopaminergic neuronal cell death is assessed by immunostaining of tyrosine hydroxylase (TH ).
