PLASTICITY AND DIFFERENTIATION OF EMBRYONIC RETINAL CELLS AFTER TERMINAL MITOSIS
PLASTICITY AND DIFFERENTIATION OF EMBRYONIC RETINAL CELLS AFTER TERMINAL MITOSIS
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DOI：
10.1126/science.2911751
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发表时间：
1989-01-20
期刊：
影响因子：
56.9
通讯作者：
HATLEE, M
中科院分区：
文献类型：
--
作者：
ADLER, R;HATLEE, M
终末有丝分裂和决定胚胎前体细胞发育命运的事件之间的关系还不清楚。这种关系现在已经调查与[3 H]胸苷放射自显影，以确定细胞出生的时间，并与培养系统，允许测试的发育潜力的细胞分离的鸡胚视网膜。与体内的情况相反，其中神经元分化总是先于感光细胞分化，感光细胞分化过早发生，并且当前体细胞在早期胚胎阶段从视网膜分离时先于神经元分化。因此，在胚胎第5天（艾德-5）出生的细胞在分离用于在艾德-6培养时主要产生光感受器，但在分离于艾德-8时主要发育为神经元。这表明视网膜前体细胞在终末有丝分裂后保留了发育为神经元或感光细胞的能力。此外，光感受器分化似乎代表了前体细胞在缺乏神经元诱导信号的情况下遵循的组成性或“默认”途径。
The relation between terminal mitosis and the events that determine the developmental fate of embryonic precursor cells is not well understood. This relation has now been investigated with [3H]thymidine autoradiography to determine the time of cell birth and with a culture system that allows the testing of the developmental potential of cells isolated from the chick embryo retina. Contrary to the situation in vivo, where neuronal differentiation always precedes photoreceptor differentiation, photoreceptor differentiation occurs prematurely and precedes neuronal differentiation when precursor cells are isolated from the retina at early embryonic stages. Thus, cells born by embryonic day 5 (ED-5) give rise predominantly to photoreceptors when isolated for culture on ED-6 but develop mainly as neurons when isolated on ED-8. This suggests that retinal precursor cells retain after terminal mitosis the capacity to develop either as neurons or as photoreceptors. Moreover, photoreceptor differentiation appears to represent a constitutive or "default" pathway that precursor cells follow in the absence of neuron-inducing signals.