Female-specific doublesex dsRNA interrupts yolk protein gene expression and reproductive ability in oriental fruit fly, Bactrocera dorsalis (Hendel)
Female-specific doublesex dsRNA interrupts yolk protein gene expression and reproductive ability in oriental fruit fly, Bactrocera dorsalis (Hendel)
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DOI：
10.1016/j.ibmb.2007.10.003
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发表时间：
2008-02-01
影响因子：
3.8
通讯作者：
Chang, Cheng
中科院分区：
文献类型：
--
作者：
Chen, Shiu-Ling;Dai, Shu-Mei;Chang, Cheng
已从东方实蝇Bactrocera dorsalis（Hendel）中克隆了doublex基因的同源物（Bddsx）。北方分析表明Bddsx在雄性和雌性果蝇中的差异表达，如对其他dsx基因所报道的。DNA结合结构域/寡聚化结构域1和寡聚化结构域2的结构保守性表明，该果蝇的doublemex蛋白（BdDSX）作为下游性别特异性基因表达的转录因子。雌性特异性转录本中推定的Transformer/Transformer-2蛋白结合序列表明，在其他果蝇中发现的保留的选择性剪接过程介导Bddsx转录本的合成。来自成人腹部dsRNA注射测定的RNA干扰（RNAi）数据表明，雌性特异性dsx dsRNA特异性地减少其自身的转录物，选择性地抑制卵黄蛋白基因（Bdypl）的表达，并延迟卵巢发育。经RNAi处理后，成熟卵的数量显著减少，但后代的性比没有出现偏差。此外，27%的雌性后代与RNAi显示畸形的产卵器，但男性苍蝇不受影响。虽然这是一个短暂的治疗，特定的Bddsx（f）干扰提供了一个有前途的和新的方法，东方果蝇控制的未来。(C)2007爱思唯尔有限公司保留所有权利。
A homologue of the doublesex gene (Bddsx) has been cloned from the oriental fruit fly, Bactrocera dorsalis (Hendel). Northern analysis indicates a differential expression of Bddsx in male and female flies, as reported for other dsx genes. A structural conservation of DNA binding domain/oligomerization domain 1 and oligomerization domain 2 suggests that the doublesex protein (BdDSX) of this fruit fly serves as a transcriptional factor for downstream sex-specific gene expression. The putative transformer/transformer-2 protein binding sequence in female-specific transcript suggests that a preserved alternative splicing process found in other flies mediates the synthesis of Bddsx transcript. RNA interference (RNAi) data from adult abdominal dsRNA injection assays indicate that female-specific dsx dsRNA reduces specifically its own transcript, inhibits selectively expression of the yolk protein gene (Bdypl), and delays ovary development. The number of matured eggs is significant reduced after RNAi treatment, but the sex ratio of offspring is not biased. Moreover, 27% of female progeny with RNAi show deformed ovipositor, but male flies are not affected. Although this is a transient treatment, the specific Bddsx(f) interference offers a promising and novel approach to oriental fruit fly control in the future. (C) 2007 Elsevier Ltd. All rights reserved.