A clinical grade cocktail of cytokines and PGE2 results in uniform maturation of human monocyte-derived dendritic cells:: implications for immunotherapy
A clinical grade cocktail of cytokines and PGE2 results in uniform maturation of human monocyte-derived dendritic cells:: implications for immunotherapy
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DOI：
10.1016/s0264-410x(02)00382-1
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发表时间：
2002-12-19
期刊：
影响因子：
5.5
通讯作者：
Bhardwaj, N
中科院分区：
文献类型：
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作者：
Lee, AW;Truong, T;Bhardwaj, N
树突状细胞（DC）可以诱导人类肿瘤或病原体特异性T细胞反应。我们全面比较了临床上可用的DC成熟刺激物促进均匀成熟DC的能力，这些DC引起更高水平的T细胞应答。我们比较了标准的成熟刺激物，自体单核细胞条件培养基（MCM），与合成的双链RNA（聚I：Q，可溶性CD 40配体三聚体，以及确定的细胞因子（TNF-α，IL-1 β，IL-6）和PGE（2）的混合物，以促进人单核细胞衍生的DC的成熟表型和功能。尽管缺乏IL-12 p70的诱导，但混合物是最有效的。虽然这些结果支持在临床DC免疫治疗试验中使用MCM模拟鸡尾酒，但其单个成分的作用仍有待完全定义。(C)2002年由Elsevier Science Ltd.出版
Dendritic cells (DCs) can induce tumor- or pathogen-specific T cell responses in humans. We comprehensively compared the clinically available DC maturation stimuli for their ability to promote uniformly mature DCs that elicit higher levels of T cell responses. We compared the standard maturation stimulus, autologous monocyte-conditioned medium (MCM), with a synthetic double stranded RNA (poly l:Q, soluble CD40 ligand trimer, and a defined cocktail of cytokines (TNF-alpha, IL-1beta, IL-6) and PGE(2) to promote mature phenotype and function in human monocyte-derived DCs. The cocktail was the most efficient despite the lack of induction of IL-12p70. While these results support the use of the MCM-mimic cocktail in clinical DC immunotherapy trials, the roles of it's individual constituents remain to be completely defined. (C) 2002 Published by Elsevier Science Ltd.