Adhesion among neural cells of the chick embryo. III. Relationship of the surface molecule CAM to cell adhesion and the development of histotypic patterns.
Adhesion among neural cells of the chick embryo. III. Relationship of the surface molecule CAM to cell adhesion and the development of histotypic patterns.
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DOI：
10.1083/jcb.79.2.371
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发表时间：
1978-11
期刊：
影响因子：
--
通讯作者：
Edelman GM
中科院分区：
文献类型：
--
作者：
Rutishauser U;Thiery JP;Brackenbury R;Edelman GM
我们以前已经确定了一个分子（命名为细胞粘附分子[CAM]），这是在鸡胚神经细胞的体外聚集参与。在本报告中，特异性抗CAM抗体已被用来证明CAM是本地化的神经组织，并与视网膜细胞和神经突起的质膜。此外，通过抗体吸收技术已经表明，最初从较老胚胎获得的培养视网膜细胞的粘附性降低与这些细胞表面上的细胞粘附分子的密度或可接近性的降低相关。CAM在神经细胞聚集中的中心作用已经通过在多种测定中观察到抗CAM Fab'片段抑制神经细胞之间的粘附而确立。为了研究CAM和细胞粘附在发育组织中的功能，在存在和不存在抗CAM Fab '的情况下培养能够在体外形成组织型模式的视网膜细胞聚集体。发现Fab'抑制细胞体和神经突的分选并减少膜-膜接触的数量，表明CAM与细胞-细胞、细胞-神经突和神经突-神经突相互作用相关。
We have previously identified a molecule (named cell adhesion molecule [CAM]) that is involved in the in vitro aggregation of neural cells from chick embryos. In the present report, specific anti-CAM antibodies have been used to demonstrated that CAM is localized in neural tissues, and is associated with the plasma membrane of retinal cells and neurites. Furthermore, it has been shown by antibody absorption techniques that the decreased adhesiveness of cultured retinal cells obtained originally from older embryos is correlated with a decrease in the density or accessibility of cell adhesion molecules on the surface of these cells. The central role of CAM in neural cell aggregation has been established by the observation that anti-CAM Fab' fragments inhibit adhesion between neural cells in a variety of assays. To investigate the function of CAM and cell adhesion in developing tissues, aggregates of retinal cells that are capable of forming histotypic patterns in vitro were cultured in the presence and absence of anti-CAM Fab'. The Fab' was found to inhibit sorting out of cell bodies and neurites and to decrease the number of membrane-membrane contacts, suggesting that CAM is associated with cell-cell, cell- neurite, and neurite-neurite interactions.