Isolation of a liver-specific promoter for human growth hormone receptor gene.
Isolation of a liver-specific promoter for human growth hormone receptor gene.
复制标题
人生长激素受体基因的肝脏特异性启动子的分离。
DOI：
10.1210/endo.138.4.5178
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发表时间：
1997
期刊：
影响因子：
4.8
通讯作者：
Mark A. Sperling
中科院分区：
文献类型：
--
作者：
L. Zou;Lynn A. Burmeister;Mark A. Sperling
生长激素（GH）的生物学作用是通过生长激素受体（GHR）介导的。GHR基因以组织特异性的方式表达，在人体组织中已检测到GHR mRNA的多种变体（V1至V8）。为了了解GHR基因的表达调控，我们分离了一个包含GHR V1、V4、V7和V8外显子5′-非翻译区（5′utr）的人类基因组克隆。在人肝癌细胞系HepG2的瞬时转染实验中，V1特异性UTR的2千碱基（kb） 5'上游序列具有启动子活性。V1变体在成人肝脏中只表达，而其他变体在该组织中缺乏表达，这表明V1 5‘ utr代表了人类GHR基因的肝脏特异性5’非编码外显子。该数据与首次分离出的人类GHR肝脏特异性启动子一致。
The biological actions of growth hormone (GH) are mediated through the growth hormone receptor (GHR). The GHR gene is expressed in a tissue specific manner and multiple variants (V1 to V8) of GHR mRNA have been detected in human tissues. To understand the regulation of GHR gene expression, a human genomic clone containing the 5'-untranslated region (5'UTR) of the V1, V4, V7 and V8 exons of the GHR was isolated. The 2 kilobase (kb) 5' upstream sequence of the V1 specific UTR has promoter activity in transient transfection assays of the human hepatoma cell line, HepG2. The exclusive expression of the V1 variant in adult liver, and the lack of expression of the other variants in this tissue, suggests that the V1 5'UTR represents the liver specific 5' noncoding exon for the human GHR gene. The data are consistent with the first isolation of a liver specific promoter for human GHR.