DOMINANT MUTATIONS (LEX) IN ESCHERICHIA-COLI K-12 WHICH AFFECT RADIATION SENSITIVITY AND FREQUENCY OF ULTRAVIOLET LIGHT-INDUCED MUTATIONS
DOMINANT MUTATIONS (LEX) IN ESCHERICHIA-COLI K-12 WHICH AFFECT RADIATION SENSITIVITY AND FREQUENCY OF ULTRAVIOLET LIGHT-INDUCED MUTATIONS
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DOI：
10.1128/jb.112.2.886-893.1972
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发表时间：
1972-01-01
影响因子：
3.2
通讯作者：
EDMISTON, SJ
中科院分区：
文献类型：
--
作者：
MOUNT, DW;LOW, KB;EDMISTON, SJ
通过三因子转导杂交，发现大肠杆菌K-12对紫外线和电离辐射敏感性增加的三个突变lex-1、lex-2和lex-3与E. coliK-12连锁图谱。携带这些突变的菌株在遗传重组中似乎没有缺陷，尽管在一些接合杂交中，它们可能无法产生正常产量的遗传重组体，这取决于交配时间和所选择的标记。突变株的紫外诱变活性降低。在用紫外线照射后，菌株的培养物以高速率降解其脱氧核糖核酸，类似于torecA−突变菌株。发现Stablelex+/lec−杂合子具有单倍体−菌株的突变辐射敏感表型。
Three mutations, denotedlex-1, -2and-3, which increase the sensitivity ofEscherichia coliK-12 to ultraviolet light (UV) and ionizing radiation, have been found by three-factor transduction crosses to be closely linked touvrAon theE. coliK-12 linkage map. Strains bearing these mutations do not appear to be defective in genetic recombination although in some conjugational crosses they may fail to produce a normal yield of genetic recombinants depending upon the time of mating and the marker selected. The mutagenic activity of UV is decreased in the mutant strains. After irradiation with UV, cultures of the strains degrade their deoxyribonucleic acid at a high rate, similar torecA−mutant strains. Stablelex+/lec−heterozygotes are found to have the mutant radiation-sensitive phenotype of haploidlex−strains.