Presence of anti-La(SS-B) is associated with binding to the 13-kD carboxyl terminus of 60-kD Ro(SS-A) in systemic lupus erythematosus.
Presence of anti-La(SS-B) is associated with binding to the 13-kD carboxyl terminus of 60-kD Ro(SS-A) in systemic lupus erythematosus.
复制标题
在系统性红斑狼疮中，抗 La(SS-B) 的存在与与 60-kD Ro(SS-A) 的 13-kD 羧基末端的结合有关。
DOI：
10.1111/1523-1747.ep12472055
复制
发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Scofield,RH
中科院分区：
文献类型：
--
作者：
Dickey,WD;vanEgmond,JE;Hardgrave,KL;Harley,JB;Scofield,RH
在系统性红斑狼疮 (SLE) 的临床表现中，自身抗体的产生和免疫遗传学是相互关联的。研究部分自身免疫反应的能力可能有助于更详细地了解这些关系。我们进行这项研究是为了确定对 60-kD Ro(SS-A) 的自身免疫反应的精细特异性是否与其他自身抗体的存在有关。我们对 74 名 SLE 患者进行了针对 60-kD Ro(SS-A) (13 kD) 羧基 13-kD 末端抗体的筛查。二十五份血清含有此类抗体。这种反应性的特点是不仅存在抗 Ro(SS-A) 抗体，还存在其他抗体。所有含有 Ro(SS-A) 和 La(SS-B) Ouchterloney 免疫扩散沉淀素的 9 份血清均结合 13-kD (p = 0.01)，而通过免疫吸附测定测定，含有抗 Ro(SS-A) 和抗 La(SS-B) 的 11 份血清中的 10 份血清结合 13-kD (p = 0.002)。抑制研究表明，结合 13-kD 片段的抗体结合 60-kD Ro(SS-A) 蛋白，但不结合 La(SS-B) 蛋白。因此，在与 60-kD Ro(SS-A) 的 13-kD 羧基末端内的表位结合的那些血清中发现了抗 La(SS-B)。这些数据表明了抗 Ro(SS-A) 和抗 La(SS-B) 在 SLE 中偶联的结构基础。
In systemic lupus erythematosus (SLE) clinical manifestations, autoantibody production, and immunogenetics are inter-related. The ability to study parts of the autoimmune response may allow a more detailed understanding of these relationships. We undertook this study to determine whether the fine specificity of the autoimmune response to 60-kD Ro(SS-A) was related to the presence of other autoantibodies. We screened 74 patients with SLE for antibodies to the carboxyl 13-kD terminal of 60-kD Ro(SS-A) (13 kD). Twenty-five sera had such antibodies. This reactivity was distinguished by the presence of not only anti-Ro(SS-A) but also other antibodies. All nine sera with Ro(SS-A) and La(SS-B) Ouchterloney immunodiffusion precipitins bound 13-kD (p = 0.01), whereas 10 of 11 sera with both anti-Ro(SS-A) and anti-La(SS-B) as determined by immunosorbent assay bound 13-kD (p = 0.002). Inhibition studies demonstrated that antibodies binding the 13-kD fragment bound the 60-kD Ro(SS-A) protein but did not bind the La(SS-B) protein. Thus, anti-La(SS-B) was found in those sera that bound epitopes within the 13-kD carboxyl terminal of 60-kD Ro(SS-A). These data suggest a structural basis by which anti-Ro(SS-A) and anti-La(SS-B) are coupled in SLE.