Steroidogenesis and prostaglandin synthesis by cultured bovine blastocysts.
Steroidogenesis and prostaglandin synthesis by cultured bovine blastocysts.
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培养牛囊胚的类固醇生成和前列腺素合成。
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
W. Hansel
中科院分区：
文献类型：
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作者：
M. Shemesh;F. Milaguir;N. Ayalon;W. Hansel
在第13、15和16天收集牛囊胚，置于添加5%胎牛血清的TCM-199中;一些囊胚立即冷冻，而另一些囊胚培养48 h后冷冻。将样品（组织+培养基，5- 12只/组）解冻，均质化，并通过放射免疫测定法进行分析。在所有囊胚中均发现了可测量的孕酮含量，但培养后的值更高（P <0.01）。在第13天，在培养的或未培养的囊胚中未发现Tetravirus，但在第15天和第16天可检测到Tetravirus，并且在培养的囊胚中含量更高（P <0.05）。PGF和PGE-2在培养3天的囊胚中均升高（P <0.05）。雌二醇在一些但不是所有囊胚中可测量。这表明，PG合成酶和酶能够合成孕酮，睾酮，并可能，雌二醇存在于这些早期牛囊胚。
Bovine blastocysts were collected at Days 13, 15 and 16 and placed in TCM-199 supplemented with 5% fetal calf serum; some blastocysts were immediately frozen while the others were cultured for 48 h and then frozen. Samples (tissue + medium, 5--12/group) were thawed, homogenized and analysed by radioimmunoassays. Measurable amounts of progesterone were found in all blastocysts but values were higher (P less than 0.01) after culture. Testosterone was not found in the cultured or uncultured blastocysts at Day 13, but was detectable on Days 15 and 16 and in greater amounts (P less than 0.05) in the cultured blastocysts. PGF and PGE-2 were increased (P less than 0.05) in the cultured blastocysts in all 3 days. Oestradiol was measurable in some but not all blastocysts. It is suggested that PG synthetase and enzymes capable of synthesizing progesterone, testosterone and, possibly, oestradiol are present in these early bovine blastocysts.