Microtubule plus-ends reveal essential links between intracellular polarization and localized modulation of endocytosis during division-plane establishment in plant cells
Microtubule plus-ends reveal essential links between intracellular polarization and localized modulation of endocytosis during division-plane establishment in plant cells
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DOI：
10.1186/1741-7007-3-11
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发表时间：
2005-04-14
期刊：
影响因子：
5.4
通讯作者：
Gadella, TWJ
中科院分区：
文献类型：
--
作者：
Dhonukshe, P;Mathur, J;Gadella, TWJ
背景：植物形态建成的一个关键事件是分裂平面的建立。一个植物特异性的微管preprophase带（PPB）准确地预测细胞分裂的线，而成膜体，另一种植物特异性阵列，执行细胞分裂，通过维持这一预测线。虽然这些特定阵列的建立显然涉及细胞内复极化事件，将细胞资源集中到分裂位点，但仍不清楚微管如何定位细胞分裂平面。在这里，我们研究了GFP-AtEB 1装饰微管加端解剖事件在division plane.Results：早期有丝分裂事件包括引导生长的内质微管（EMT）对PPB网站和他们的一致本地化与内吞囊泡。因此，内体带位于微管PPB在其成熟，并在纺锤体形成过程中保持密切的接近。在胞质分裂过程中，EMT从前纺锤体极以与细胞板导航和倾斜校正相关的几何构型辐射。Naphthylphtalamic acid（NPA），极性生长素外排的抑制剂，引起异常PPBs和移位分裂planes.Conclusion：我们的观察揭示了微管和细胞内极化之间的时空联系本地化的内吞作用和精确的建立在植物中的分裂平面。此外，他们牵连的生长调节剂，生长素，在这一重要的细胞事件。
Background: A key event in plant morphogenesis is the establishment of a division plane. A plant-specific microtubular preprophase band (PPB) accurately predicts the line of cell division, whereas the phragmoplast, another plant-specific array, executes cell division by maintaining this predicted line. Although establishment of these specific arrays apparently involves intracellular repolarization events that focus cellular resources to a division site, it still remains unclear how microtubules position the cell division planes. Here we study GFP-AtEB1 decorated microtubule plus-ends to dissect events at the division plane.Results: Early mitotic events included guided growth of endoplasmic microtubules (EMTs) towards the PPB site and their coincident localization with endocytic vesicles. Consequently, an endosomal belt lay in close proximity to the microtubular PPB at its maturation and was maintained during spindle formation. During cytokinesis, EMTs radiated from the former spindle poles in a geometrical conformation correlating with cell-plate navigation and tilt-correction. Naphthylphtalamic acid (NPA), an inhibitor of polar auxin efflux, caused abnormal PPBs and shifted division planes.Conclusion: Our observations reveal a spatio-temporal link between microtubules and intracellular polarization essential for localized endocytosis and precise establishment of the division plane in plants. Additionally, they implicate the growth regulator, auxin, in this important cellular event.