Overexpression of oncoprotein 18 correlates with poor differentiation in lung adenocarcinomas
Overexpression of oncoprotein 18 correlates with poor differentiation in lung adenocarcinomas
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DOI：
10.1074/mcp.m200055-mcp200
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发表时间：
2003-02-01
影响因子：
7
通讯作者：
Beer, DG
中科院分区：
文献类型：
--
作者：
Chen, G;Wang, H;Beer, DG
我们检测了93例肺腺癌和10例未受累肺标本中癌蛋白18（Op18）的表达，采用定量二维PAGE分析，并通过质谱和二维Western印迹分析进行确认。使用寡核苷酸微阵列检测mRNA表达，使用组织微阵列的免疫组织化学分析检测Op18蛋白的细胞定位。三种磷酸化形式和一种非磷酸化形式的Op18蛋白被鉴定，并发现在肺腺癌中过表达与正常肺相比。磷酸化占总Op18蛋白亚型的百分比从正常肺中的3.2%增加到肺肿瘤中的7.9%。与中、高分化肺腺癌相比，低分化肺腺癌中磷酸化和非磷酸化Op18蛋白均显著增加（p
We examined the expression of oncoprotein 18 (Op18) in 93 lung adenocarcinomas and 10 uninvolved lung samples using quantitative two-dimensional PAGE analysis with confirmation by mass spectrometry and two-dimensional Western blot analysis. mRNA expression was examined using oligonucleotide microarrays, and the cellular localization of the Op18 protein was examined using immunohistochemical analysis of tissue microarrays. Three phosphorylated forms and one unphosphorylated form of the Op18 protein were identified and found to be overexpressed in lung adenocarcinomas as compared with normal lung. The percentage of phosphorylated to total Op18 protein isoforms increased from 3.2% in normal lung to 7.9% in lung tumors. Both the phosphorylated and unphosphorylated Op18 proteins were significantly increased in poorly differentiated tumors as compared with moderately or well differentiated lung adenocarcinomas (p