DNA strand separation studied by single molecule force measurements
DNA strand separation studied by single molecule force measurements
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DOI：
10.1103/physreve.58.2386
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发表时间：
1998-08-01
影响因子：
2.4
通讯作者：
Heslot, F
中科院分区：
文献类型：
--
作者：
Bockelmann, U;Essevaz-Roulet, B;Heslot, F
我们将DNA双螺旋一端的两条互补链分别连接到玻璃载玻片和玻璃微针上。将滑动片从针上移开，分子逐渐被拉开，针的挠度变化使打开力发生相应的变化。力的信号是非常丰富的细节可重复获得。在20 nm/s ~ 800 nm/s范围内，力信号的平均水平和幅值几乎与打开速度无关。基于热平衡假设的理论描述使我们能够将测得的力曲线与DNA的基因组序列联系起来。揭示了分子粘滑运动，与宏观固体摩擦动力学相反，它是一个确定性和可重复的过程。这一过程在实验理论上得到了考虑。
We have separately attached the two complementary strands of one end of a DNA double helix to a glass slide and a glass microneedle. Displacing the slide away from the needle, the molecule is progressively pulled open and the changing deflection of the needle gives the corresponding variation in the opening force. Force signals which are very rich in detail are reproducibly obtained. The average level and amplitude of the force signal is almost independent of the opening velocity in the interval 20 nm/s to 800 nm/s. A theoretical description based on the assumption of thermal equilibrium allows us to link the measured force curves to the genomic sequence of the DNA. A molecular stick-slip motion is revealed, which in contrast to the dynamics of macroscopic solid friction is a deterministic and reproducible process. This process is considered experimentally theoretically.