Increased expression of triggering receptor expressed on myeloid cells-1 in the population with obesity and insulin resistance.
Increased expression of triggering receptor expressed on myeloid cells-1 in the population with obesity and insulin resistance.
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DOI：
10.1002/oby.21714
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发表时间：
2017-03
期刊：
影响因子：
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通讯作者：
Nandipati KC
中科院分区：
文献类型：
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作者：
Subramanian S;Pallati PK;Rai V;Sharma P;Agrawal DK;Nandipati KC
髓样细胞表达的触发受体（TREM）-1最近被认为是急性和慢性炎症的有效放大器之一。然而，TREM-1在胰岛素不敏感性方面的确切作用是未知的。我们检测了组织活检中TREM-1、TREM-2和TREM-1/TREM-2比值的mRNA转录和蛋白表达（肝脏、网膜和皮下脂肪）和血液样本（中性粒细胞和单核细胞）（SO+D+; n=15），肥胖但无糖尿病的受试者（SO+D-; n=7），并与无肥胖（BMI < 30）和糖尿病的受试者（SO-D-; n=5）进行比较。免疫荧光和RT-PCR显示SO+D+组与其他组相比，TREM-1显著增加，TREM-2显著减少，TREM 1/TREM 2比值显著增加。总体而言，SO+D+组与SO+D−组相比，肝脏TREM-1表达和可溶性TREM-1增加（100% vs 57.14%，r=0.582; P=0.023）。TREM-1在HOMA-IR指数>2的所有肥胖受试者中显著升高。发现TREM-1在肥胖受试者的组织活检和血液中显著更高。TREM-1的更高表达和活性表明在肥胖和相关共病的潜在病理生理学中可能起作用。
Triggering receptor expressed on myeloid cells (TREM)-1 has recently been recognized as one of the potent amplifiers of acute and chronic inflammation. However, exact role of TREM-1 in regard to insulin insensitivity is unknown. We examined the mRNA transcripts and protein expression of TREM-1, TREM-2 and TREM-1/TREM-2 ratio in the tissue biopsies (liver, omentum and subcutaneous fat) and blood samples (neutrophils and monocytes) of subjects with obesity and diabetes (SO+D+; n=15), subjects with obesity but not diabetes (SO+D−; n=7), and compared with the subjects without obesity (BMI < 30) and diabetes (SO−D−; n=5). The immunofluorescence and RT-PCR revealed significant increase in TREM-1, decrease in TREM-2, and increase in the TREM1/TREM2 ratio in SO+D+ group compared to other groups. Overall, increased liver TREM-1 expression and soluble-TREM-1 were found in SO+D+ group compared to SO+D− group (100% vs 57.14%, r=0.582; P=0.023). TREM-1 was significantly increased in all subjects with obesity, those had HOMA-IR index of >2. TREM-1 was found to be significantly higher in tissues biopsies and blood of subjects with obesity. Greater expression and activity of TREM-1 suggests a possible role in the underlying pathophysiology of obesity and associated co-morbidities.