Characterization of secreted proteases of Paenibacillus larvae, potential virulence factors involved in honeybee larval infection
Characterization of secreted proteases of Paenibacillus larvae, potential virulence factors involved in honeybee larval infection
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DOI：
10.1016/j.jip.2009.07.010
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发表时间：
2009-10-01
影响因子：
3.4
通讯作者：
Zunino, Pablo
中科院分区：
文献类型：
--
作者：
Antunez, Karina;Anido, Matilde;Zunino, Pablo
类芽孢杆菌幼虫是美国幼虫腐臭病 (AFB) 的病原体，这是影响蜜蜂幼虫的最严重的细菌性疾病。 AFB 导致蜜蜂数量大幅减少，影响养蜂业和农业生产。幼虫感染后，P.幼虫会分泌可能参与致病性的蛋白酶。在本文中，我们介绍了 P. 幼虫分泌的不同蛋白酶。抑制测定证实了金属蛋白酶的存在。在来自不同地理来源的 P. larvae 分离物集合中鉴定出两种不同的蛋白酶模式（PP1 和 PP2）。 49% 的 P. larvae 分离株表现出 PP1 模式，而 51% 表现出 PP2 模式。大多数属于基因型 ERIC I - BOX A 的分离株呈现 PP2，大多数属于 ERIC I - BOX C 的分离株呈现 PP1，但相关性并不显着。属于基因型 ERIC II 和 ERIC III 的分离株呈 PP2。分泌蛋白酶模式与地理分布之间没有观察到相关性，因为这两种模式在乌拉圭广泛分布。根据暴露生物测定，显示 PP2 的分离株比显示 PP1 的分离株毒力更强，表明致病性的差异可能与蛋白酶的分泌有关。 (C) 2009 Elsevier Inc. 保留所有权利。
Paenibacillus larvae is the causative agent of American Foulbrood (AFB), the most severe bacterial disease that affects honeybee larvae. AFB causes a significant decrease in the honeybee population affecting the beekeeping industry and agricultural production. After infection of larvae, P. larvae secretes proteases that could be involved in the pathogenicity. In the present article, we present the secretion of different proteases by P. larvae. Inhibition assays confirmed the presence of metalloproteases. Two different proteases patterns (PP1 and PP2) were identified in a collection of P. larvae isolates from different geographic origin. Forty nine percent of P. larvae isolates showed pattern PP1 while 51% exhibited pattern PP2. Most isolates belonging to genotype ERIC I - BOX A presented PP2, most isolates belonging to ERIC I - BOX C presented PP1 although relations were not significant. Isolates belonging to genotypes ERIC II and ERIC III presented PP2. No correlation was observed between the secreted proteases patterns and geographic distribution, since both patterns are widely distributed in Uruguay. According to exposure bioassays, isolates showing PP2 are more virulent than those showing PP1, suggesting that difference in pathogenicity could be related to the secretion of proteases. (C) 2009 Elsevier Inc. All rights reserved.