PESTIVIRUSES ISOLATED FROM PIGS, CATTLE AND SHEEP CAN BE ALLOCATED INTO AT LEAST 3 GENOGROUPS USING POLYMERASE CHAIN-REACTION AND RESTRICTION-ENDONUCLEASE ANALYSIS
PESTIVIRUSES ISOLATED FROM PIGS, CATTLE AND SHEEP CAN BE ALLOCATED INTO AT LEAST 3 GENOGROUPS USING POLYMERASE CHAIN-REACTION AND RESTRICTION-ENDONUCLEASE ANALYSIS
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DOI：
10.1007/bf01321060
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发表时间：
1994-01-01
影响因子：
2.7
通讯作者：
PATON, DJ
中科院分区：
文献类型：
--
作者：
VILCEK, S;HERRING, AJ;PATON, DJ
开发了一种基于聚合酶链反应的检测方法，能够检测来自猪、牛或羊的多种鼠疫病毒。从鼠疫病毒基因组的不同部分选择的6组引物中，高度保守的5 '非编码区引物对129株分离物的扩增效果最好。该小组包括33株猪分离株、79株牛分离株和17株羊分离株。用限制性内切酶AvaI和BglI切割pcr扩增产物，实现了病毒之间的分化。使用这种方法可以区分至少3个基因组；第1组（HCV）有32株猪分离株，第2组（BVDV）有所有牛分离株和6株羊分离株，第3组（BDV）有11株羊分离株和1株猪分离株。
A polymerase chain reaction-based assay capable of detecting a broad range of pestiviruses from pigs, cattle, or sheep was developed. Of six sets of primers selected from different parts of the pestivirus genome, the best results were provided by a pair from the highly conserved 5 ' non-coding region which gave amplification with all 129 isolates tested. This panel consisted of 33 isolates from pigs, 79 from cattle, and 17 from sheep. Differentiation between the viruses was achieved by cutting the PCR-amplified products with the restriction endonucleases AvaI and BglI. Using this procedure it was possible to distinguish at least 3 genogroups; group 1 (HCV) contained 32 of the pig isolates, group II (BVDV) contained all the cattle isolates tested plus 6 sheep isolates and group III (BDV) contained 11 sheep isolates and 1 pig isolate.